{"created":"2023-05-15T14:38:00.314808+00:00","id":49026,"links":{},"metadata":{"_buckets":{"deposit":"8c98f049-42d8-4cd5-a004-74e0e3004e64"},"_deposit":{"created_by":1,"id":"49026","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49026"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049026","sets":["1"]},"author_link":["494439","494444","494438","494441","494446","494436","494442","494445","494443","494440","494437"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"25","bibliographicPageStart":"21","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"Nuclear Medicine and Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Abstract\nIntroduction\nDetection of vulnerable plaques is critically important for the selection of appropriate treatment and/or the prevention of atherosclerosis and ensuing cardiovascular diseases. In order to clarify the utility of [11C]acetate for atherosclerosis imaging, we determined the uptake and metabolism of acetate by in vitro studies using rabbit atherosclerotic arteries and [14C]acetate.\nMethods\nRabbits were fed with a conventional (n = 5) or a 0.5% cholesterol diet (n = 6). One side of the iliac–femoral arteries was injured by a balloon catheter. Radioactivity levels in the iliac–femoral arteries were measured after incubation in DMEM containing [1-14C]acetate for 60 min (% dpm/mg tissue). Radioactive components in the homogenized arteries were partitioned into aqueous, organic, and residue fractions by the Folch method, and analyzed by thin-layer chromatography (TLC).\nResults\nThe radioactivity level in the injured arteries of rabbits fed with the 0.5% cholesterol diet (atherosclerotic arteries) was significantly higher than that in either the non-injured or injured arteries of rabbits fed with the conventional diet (p < 0.05) (% dpm/mg tissue: conventional diet groups; 0.022 ± 0.005 and 0.024 ± 0.007, cholesterol diet groups; 0.029 ± 0.007 and 0.034 ± 0.005 for non-injured and injured arteries). In metabolite analysis, most of the radioactivity was found in the aqueous fraction in each group (87.4–94.6% of total radioactivity in the arteries), and glutamate was a dominant component (67.4–69.7% of the aqueous fraction in the arteries).\nConclusions\nThe level of [14C]acetate-derived radioactivity into the arteries was increased by balloon injury and the burden of a cholesterol diet. Water-soluble metabolites were the dominant components with radioactivity in the atherosclerotic lesions. These results provide a biological basis for imaging atherosclerotic lesions by PET using [11C]acetate.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ELSEVIER"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"29055850","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.nucmedbio.2017.08.003","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.1016/j.nucmedbio.2017.08.003"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.nucmedbio.2017.08.003","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamasaki, Kazuaki"}],"nameIdentifiers":[{"nameIdentifier":"494436","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamashita, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"494437","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhao, Yan"}],"nameIdentifiers":[{"nameIdentifier":"494438","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Yoichi"}],"nameIdentifiers":[{"nameIdentifier":"494439","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishii, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"494440","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawai, Keiichi"}],"nameIdentifiers":[{"nameIdentifier":"494441","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tamaki, Nagara"}],"nameIdentifiers":[{"nameIdentifier":"494442","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhao, Songji"}],"nameIdentifiers":[{"nameIdentifier":"494443","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Asada, Yujiro"}],"nameIdentifiers":[{"nameIdentifier":"494444","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kuge, Yuji"}],"nameIdentifiers":[{"nameIdentifier":"494445","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西井 龍一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"494446","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"In vitro uptake and metabolism of [14C]acetate in rabbit atherosclerotic arteries: biological basis for atherosclerosis imaging with [11C]acetate","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In vitro uptake and metabolism of [14C]acetate in rabbit atherosclerotic arteries: biological basis for atherosclerosis imaging with [11C]acetate"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-05-17"},"publish_date":"2018-05-17","publish_status":"0","recid":"49026","relation_version_is_last":true,"title":["In vitro uptake and metabolism of [14C]acetate in rabbit atherosclerotic arteries: biological basis for atherosclerosis imaging with [11C]acetate"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:21:46.327590+00:00"}