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Validation of 64Cu-ATSM damaging DNA via high-LET Auger electron emission
https://repo.qst.go.jp/records/47406
https://repo.qst.go.jp/records/47406e5cbc038-43a1-48e1-88de-77e21b5e27ee
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-05-18 | |||||
| タイトル | ||||||
| タイトル | Validation of 64Cu-ATSM damaging DNA via high-LET Auger electron emission | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
D., McMillan Dayton
× D., McMillan Dayton× Maeda, Junko× Justin, Bell× D., Genet Matthew× Phoonswadi, Garrett× A., Mann Kelly× L., Kraft Susan× Kitamura, Hisashi× Fujimori, Akira× Yoshii, Yukie× Furukawa, Takako× Fujibayashi, Yasuhisa× Kato, Takamitsu× Ｊｕｓｔｉｎ Ｂｅｌｌ× 北村 尚× 藤森 亮× 吉井 幸恵× 古川 高子× 藤林 康久× 加藤 宝光 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Radioactive copper (II) (diacetyl-bis N4-methylthiosemicarbazone) (Cu-ATSM) isotopes were originally developed for the imaging of hypoxia in tumors. Because the decay of a 64Cu atom is emitting not only positrons but also Auger electrons, this radionuclide has great potential as a theranostic agent. However, the success of 64Cu-ATSM internal radiation therapy would depend on the contribution of Auger electrons to tumor cell killing. Therefore, we designed a cell culture system to define the contributions to cell death from Auger electrons to support or refute our hypothesis that the majority of cell death from 64Cu-ATSM is a result of high-LET Auger electrons and not positrons or other low-LET radiation. Chinese hamster ovary (CHO) wild type and DNA repair–deficient xrs5 cells were exposed to 64Cu-ATSM during hypoxic conditions. Surviving fractions were compared with those surviving gamma-radiation, low-LET hadron radiation, and high-LET heavy ion exposure. The ratio of the D10 values (doses required to achieve 10% cell survival) between CHO wild type and xrs5 cells suggested that 64Cu-ATSM toxicity is similar to that of high-LET Carbon ion radiation (70 keV/μm). γH2AX foci assays confirmed DNA double-strand breaks and cluster damage by high-LET Auger electrons from 64Cu decay, and complex types of chromosomal aberrations typical of high-LET radiation were observed after 64Cu-ATSM exposure. The majority of cell death was caused by high-LET radiation. This work provides strong evidence that 64Cu-ATSM damages DNA via high-LET Auger electrons, supporting further study and consideration of 64Cu-ATSM as a cancer treatment modality for hypoxic tumors. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 56, 号 5, p. 784-791, 発行日 2015-08 |
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| 出版者 | ||||||
| 出版者 | Oxford University Press | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1093/jrr/rrv042 | |||||
