{"created":"2023-05-15T14:36:47.678571+00:00","id":47406,"links":{},"metadata":{"_buckets":{"deposit":"1f7b3aab-85c0-47ac-9249-814352c67d07"},"_deposit":{"created_by":1,"id":"47406","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"47406"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00047406","sets":["1"]},"author_link":["474661","474670","474669","474662","474668","474658","474672","474660","474664","474666","474667","474657","474663","474665","474671","474673","474674","474676","474675","474659"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"791","bibliographicPageStart":"784","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Radioactive copper (II) (diacetyl-bis N4-methylthiosemicarbazone) (Cu-ATSM) isotopes were originally developed for the imaging of hypoxia in tumors. Because the decay of a 64Cu atom is emitting not only positrons but also Auger electrons, this radionuclide has great potential as a theranostic agent. However, the success of 64Cu-ATSM internal radiation therapy would depend on the contribution of Auger electrons to tumor cell killing. Therefore, we designed a cell culture system to define the contributions to cell death from Auger electrons to support or refute our hypothesis that the majority of cell death from 64Cu-ATSM is a result of high-LET Auger electrons and not positrons or other low-LET radiation. Chinese hamster ovary (CHO) wild type and DNA repair–deficient xrs5 cells were exposed to 64Cu-ATSM during hypoxic conditions. Surviving fractions were compared with those surviving gamma-radiation, low-LET hadron radiation, and high-LET heavy ion exposure. The ratio of the D10 values (doses required to achieve 10% cell survival) between CHO wild type and xrs5 cells suggested that 64Cu-ATSM toxicity is similar to that of high-LET Carbon ion radiation (70 keV/μm). γH2AX foci assays confirmed DNA double-strand breaks and cluster damage by high-LET Auger electrons from 64Cu decay, and complex types of chromosomal aberrations typical of high-LET radiation were observed after 64Cu-ATSM exposure. The majority of cell death was caused by high-LET radiation. This work provides strong evidence that 64Cu-ATSM damages DNA via high-LET Auger electrons, supporting further study and consideration of 64Cu-ATSM as a cancer treatment modality for hypoxic tumors.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jrr/rrv042","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"D., McMillan Dayton"}],"nameIdentifiers":[{"nameIdentifier":"474657","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maeda, Junko"}],"nameIdentifiers":[{"nameIdentifier":"474658","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Justin, Bell"}],"nameIdentifiers":[{"nameIdentifier":"474659","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"D., Genet Matthew"}],"nameIdentifiers":[{"nameIdentifier":"474660","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Phoonswadi, Garrett"}],"nameIdentifiers":[{"nameIdentifier":"474661","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"A., Mann Kelly"}],"nameIdentifiers":[{"nameIdentifier":"474662","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"L., Kraft Susan"}],"nameIdentifiers":[{"nameIdentifier":"474663","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kitamura, Hisashi"}],"nameIdentifiers":[{"nameIdentifier":"474664","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"474665","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshii, Yukie"}],"nameIdentifiers":[{"nameIdentifier":"474666","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Takako"}],"nameIdentifiers":[{"nameIdentifier":"474667","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujibayashi, Yasuhisa"}],"nameIdentifiers":[{"nameIdentifier":"474668","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kato, Takamitsu"}],"nameIdentifiers":[{"nameIdentifier":"474669","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｊｕｓｔｉｎ Ｂｅｌｌ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474670","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"北村 尚","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474671","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474672","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉井 幸恵","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474673","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古川 高子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474674","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤林 康久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474675","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"加藤 宝光","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474676","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Validation of 64Cu-ATSM damaging DNA via high-LET Auger electron emission","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Validation of 64Cu-ATSM damaging DNA via high-LET Auger electron emission"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-05-18"},"publish_date":"2016-05-18","publish_status":"0","recid":"47406","relation_version_is_last":true,"title":["Validation of 64Cu-ATSM damaging DNA via high-LET Auger electron emission"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:40:14.320721+00:00"}