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The glutathione dependent reduction of a nitroxyl radical, 4-hydroxyl-2,2,6,6-tetramethylpiperidineNoxyl, which was restricted by adding superoxide dismutase or by deoxygenating the reaction mixture,\nwas applied to an index of superoxide (O2•−) generation. A reaction\nmixture containing 0.1 mM 4hydroxyl2,2,6,6tetramethylpiperidine-N-oxyl and 1 mM glutathione was prepared using 100 mM phosphate buffer containing 0.05 mM diethylenetriaminepenta acetic acid. The reaction mixture was kept in a screwtop vial and incubated in a water bath at 37 or 44°C. The time course of the electron paramagnetic resonance signal of 4-hydroxyl-2,2,6,6-tetramethylpiperidineNoxyl in the reaction mixture was measured by an X-band EPR spectrometer (JEOL, Tokyo, Japan). When the same experiment was performed using rat plasma instead of 100 mM PB, the glutathionedependent reduction of 4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl, i.e., generation of O2•−, was not obtained. 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Scavenging of reactive oxygen species Induced by hyperthermia in biological fluid
https://repo.qst.go.jp/records/46950
https://repo.qst.go.jp/records/46950b5326472-748c-4f81-bbc1-c13a9c3ff7c1
名前 / ファイル | ライセンス | アクション |
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Ueno.M.2014.J.Clin.Biochem.Nutr.54.75-80.pdf (778.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-02-14 | |||||
タイトル | ||||||
タイトル | Scavenging of reactive oxygen species Induced by hyperthermia in biological fluid | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ueno, Megumi
× Ueno, Megumi× Nyui, Minako× Nakanishi, Ikuo× Anzai, Kazunori× Ozawa, Toshihiko× Ken-ichiro, Matsumoto× Uto, Yoshihiro× Megumi, Ueno× Minako, Nyui× Ikuo, Nakanishi× Kazunori, Anzai× Toshihiko, Ozawa× Kenichiro, Matsumoto |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The scavenging activity of rat plasma against hyperthermia-induced reactive oxygen species was tested. The glutathione dependent reduction of a nitroxyl radical, 4-hydroxyl-2,2,6,6-tetramethylpiperidineNoxyl, which was restricted by adding superoxide dismutase or by deoxygenating the reaction mixture, was applied to an index of superoxide (O2•−) generation. A reaction mixture containing 0.1 mM 4hydroxyl2,2,6,6tetramethylpiperidine-N-oxyl and 1 mM glutathione was prepared using 100 mM phosphate buffer containing 0.05 mM diethylenetriaminepenta acetic acid. The reaction mixture was kept in a screwtop vial and incubated in a water bath at 37 or 44°C. The time course of the electron paramagnetic resonance signal of 4-hydroxyl-2,2,6,6-tetramethylpiperidineNoxyl in the reaction mixture was measured by an X-band EPR spectrometer (JEOL, Tokyo, Japan). When the same experiment was performed using rat plasma instead of 100 mM PB, the glutathionedependent reduction of 4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl, i.e., generation of O2•−, was not obtained. Only the firstorder decay reduction of 4hydroxyl 2,2,6,6tetramethylpiperidineNoxyl, which indicates direct reduction of 4hydroxyl2,2,6,6tetramethylpiperidineNoxyl, was obtained in rat plasma. Adding 0.5% albumin to the phosphate buffer reaction mixture could almost completely inhibit O2•− generation at 37°C. However, addition of 0.5% albumin could not inhibit O2•− generation at 44°C, i.e., hyperthermic temperature. Ascorbic acid also showed inhibition of O2•− generation by 0.01 mM at 37°C, but 0.02 mM or more could inhibit O2•− generation at 44°C. A higher concentration of ascorbic acid showed first order reduction, i.e., direct oneelectron reduction, of TEMPOL. Hyperthermiainduced O2 •− generation in rat plasma can be mostly inhibited by albumin and ascorbic acid in the plasma. |
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書誌情報 |
Journal of Clinical Biochemistry and Nutrition 巻 54, 号 2, p. 75-80, 発行日 2014-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0912-0009 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 24688214 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3164/jcbn.13-61 | |||||
関連サイト | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.3164/jcbn.13-61 |