{"created":"2023-05-15T14:36:27.588053+00:00","id":46950,"links":{},"metadata":{"_buckets":{"deposit":"62fef09c-d92b-494d-a7fb-87346abe6aa6"},"_deposit":{"created_by":1,"id":"46950","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46950"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046950","sets":["1"]},"author_link":["1024766","1024773","1024767","1024769","1024775","1024771","1024772","1024768","1024770","1024776","1024764","1024765","1024774"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"80","bibliographicPageStart":"75","bibliographicVolumeNumber":"54","bibliographic_titles":[{"bibliographic_title":"Journal of Clinical Biochemistry and Nutrition"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The scavenging activity of rat plasma against hyperthermia-induced reactive oxygen species was tested. The glutathione dependent reduction of a nitroxyl radical, 4-hydroxyl-2,2,6,6-tetramethylpiperidineNoxyl, which was restricted by adding superoxide dismutase or by deoxygenating the reaction mixture,\nwas applied to an index of superoxide (O2•−) generation. A reaction\nmixture containing 0.1 mM 4hydroxyl2,2,6,6tetramethylpiperidine-N-oxyl and 1 mM glutathione was prepared using 100 mM phosphate buffer containing 0.05 mM diethylenetriaminepenta acetic acid. The reaction mixture was kept in a screwtop vial and incubated in a water bath at 37 or 44°C. The time course of the electron paramagnetic resonance signal of 4-hydroxyl-2,2,6,6-tetramethylpiperidineNoxyl in the reaction mixture was measured by an X-band EPR spectrometer (JEOL, Tokyo, Japan). When the same experiment was performed using rat plasma instead of 100 mM PB, the glutathionedependent reduction of 4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl, i.e., generation of O2•−, was not obtained. Only the firstorder decay reduction of 4hydroxyl\n2,2,6,6tetramethylpiperidineNoxyl, which indicates direct reduction of 4hydroxyl2,2,6,6tetramethylpiperidineNoxyl, was obtained in rat plasma. Adding 0.5% albumin to the phosphate buffer reaction mixture could almost completely inhibit O2•− generation at 37°C. However, addition of 0.5% albumin could not\ninhibit O2•− generation at 44°C, i.e., hyperthermic temperature. Ascorbic acid also showed inhibition of O2•− generation by 0.01 mM at 37°C, but 0.02 mM or more could inhibit O2•− generation at 44°C. A higher concentration of ascorbic acid showed first order reduction, i.e., direct oneelectron reduction, of TEMPOL.\nHyperthermiainduced O2\n•− generation in rat plasma can be mostly\ninhibited by albumin and ascorbic acid in the plasma.","subitem_description_type":"Abstract"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"24688214","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3164/jcbn.13-61","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3164/jcbn.13-61","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0912-0009","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ueno, Megumi"}],"nameIdentifiers":[{"nameIdentifier":"1024764","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nyui, Minako"}],"nameIdentifiers":[{"nameIdentifier":"1024765","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakanishi, Ikuo"}],"nameIdentifiers":[{"nameIdentifier":"1024766","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Anzai, Kazunori"}],"nameIdentifiers":[{"nameIdentifier":"1024767","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ozawa, Toshihiko"}],"nameIdentifiers":[{"nameIdentifier":"1024768","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ken-ichiro, Matsumoto"}],"nameIdentifiers":[{"nameIdentifier":"1024769","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Uto, Yoshihiro"}],"nameIdentifiers":[{"nameIdentifier":"1024770","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Megumi, Ueno","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1024771","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Minako, Nyui","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1024772","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ikuo, Nakanishi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1024773","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kazunori, Anzai","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1024774","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Toshihiko, Ozawa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1024775","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kenichiro, Matsumoto","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1024776","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-28"}],"displaytype":"detail","filename":"ff1aa93b6f4e297d6935558dbd50573b.pdf","filesize":[{"value":"778.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Ueno.M.2014.J.Clin.Biochem.Nutr.54.75-80.pdf","url":"https://repo.qst.go.jp/record/46950/files/ff1aa93b6f4e297d6935558dbd50573b.pdf"},"version_id":"3b22bd3c-78a3-460a-95d5-6b6820ed4935"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Scavenging of reactive oxygen species Induced by hyperthermia in biological fluid","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Scavenging of reactive oxygen species Induced by hyperthermia in biological fluid"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-02-14"},"publish_date":"2014-02-14","publish_status":"0","recid":"46950","relation_version_is_last":true,"title":["Scavenging of reactive oxygen species Induced by hyperthermia in biological fluid"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:05:03.941835+00:00"}