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High linear-energy-transfer radiation can overcome radioresistance of glioma stem-like cells to low linear-energy-transfer radiation
https://repo.qst.go.jp/records/46948
https://repo.qst.go.jp/records/46948fa4a7898-f8fd-4ba5-b183-535a7e4bf40d
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-11-17 | |||||
| タイトル | ||||||
| タイトル | High linear-energy-transfer radiation can overcome radioresistance of glioma stem-like cells to low linear-energy-transfer radiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hirota, Yuki
× Hirota, Yuki× Masunaga, Shin-Ichiro× 近藤, 夏子× Kawabata, Shinji× 平川, 博一× 矢島, 浩彦× 藤森, 亮× 小野, 公二× Kuroiwa, Toshihiko× Miyatake, Shin-Ichi× 近藤 夏子× 平川 博一× 矢島 浩彦× 藤森 亮× 小野 公二 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Ionizing radiation is applied as the standard treatment for glioblastoma multiforme (GBM). However, radio- therapy remains merely palliative, not curative, because of the existence of glioma stem cells (GSCs), which are regarded as highly radioresistant to low linear-energy-transfer (LET) photons. Here we analyzed whether or not high-LET particles can overcome the radioresistance of GSCs. Glioma stem-like cells (GSLCs) were induced from the GBM cell line A172 in stem cell culture medium. The phenotypes of GSLCs and wild-type cells were confirmed using stem cell markers. These cells were irradiated with 60Co gamma rays or reactor neutron beams. Under neutron-beam irradiation, high-LET proton particles can be produced through elastic scattering or nitrogen capture reaction. Radiosensitivity was assessed by a colony-forming assay, and the DNA double-strand breaks (DSBs) were assessed by a histone gamma-H2AX focus detection assay. In stem cell culture medium, GSLCs could form neurosphere-like cells and express neural stem cell markers (Sox2 and Musashi) abundantly in comparison with their parental cells. GSLCs were significantly more radioresis- tant to gamma rays than their parental cells, but neutron beams overcame this resistance. There were signifi- cantly fewer gamma-H2AX foci in the A172 GSLCs 24 h after irradiation with gamma rays than in their parental cultured cells, while there was no apparent difference following neutron-beam irradiation. High-LET radiation can overcome the radioresistance of GSLCs by producing unrepairable DNA DSBs. High-LET radiation therapy might have the potential to overcome GBM’s resistance to X-rays in a clinical setting. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 55, 号 1, p. 75-83, 発行日 2014-01 |
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