{"created":"2023-05-15T14:36:27.492007+00:00","id":46948,"links":{},"metadata":{"_buckets":{"deposit":"8e3324c2-d1ca-4f4f-9612-35526f5c76aa"},"_deposit":{"created_by":1,"id":"46948","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46948"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046948","sets":["1"]},"author_link":["468520","468512","468511","468517","468510","468518","468506","468519","468508","468514","468516","468509","468513","468507","468515"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"83","bibliographicPageStart":"75","bibliographicVolumeNumber":"55","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ionizing radiation is applied as the standard treatment for glioblastoma multiforme (GBM). However, radio- therapy remains merely palliative, not curative, because of the existence of glioma stem cells (GSCs), which are regarded as highly radioresistant to low linear-energy-transfer (LET) photons. Here we analyzed whether or not high-LET particles can overcome the radioresistance of GSCs. Glioma stem-like cells (GSLCs) were induced from the GBM cell line A172 in stem cell culture medium. The phenotypes of GSLCs and wild-type cells were confirmed using stem cell markers. These cells were irradiated with 60Co gamma rays or reactor neutron beams. Under neutron-beam irradiation, high-LET proton particles can be produced through elastic scattering or nitrogen capture reaction. Radiosensitivity was assessed by a colony-forming assay, and the DNA double-strand breaks (DSBs) were assessed by a histone gamma-H2AX focus detection assay. In stem cell culture medium, GSLCs could form neurosphere-like cells and express neural stem cell markers (Sox2 and Musashi) abundantly in comparison with their parental cells. GSLCs were significantly more radioresis- tant to gamma rays than their parental cells, but neutron beams overcame this resistance. There were signifi- cantly fewer gamma-H2AX foci in the A172 GSLCs 24 h after irradiation with gamma rays than in their parental cultured cells, while there was no apparent difference following neutron-beam irradiation. High-LET radiation can overcome the radioresistance of GSLCs by producing unrepairable DNA DSBs. High-LET radiation therapy might have the potential to overcome GBM’s resistance to X-rays in a clinical setting.","subitem_description_type":"Abstract"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirota, Yuki"}],"nameIdentifiers":[{"nameIdentifier":"468506","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Masunaga, Shin-Ichiro"}],"nameIdentifiers":[{"nameIdentifier":"468507","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"近藤, 夏子"}],"nameIdentifiers":[{"nameIdentifier":"468508","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawabata, Shinji"}],"nameIdentifiers":[{"nameIdentifier":"468509","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平川, 博一"}],"nameIdentifiers":[{"nameIdentifier":"468510","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"矢島, 浩彦"}],"nameIdentifiers":[{"nameIdentifier":"468511","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森, 亮"}],"nameIdentifiers":[{"nameIdentifier":"468512","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小野, 公二"}],"nameIdentifiers":[{"nameIdentifier":"468513","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kuroiwa, Toshihiko"}],"nameIdentifiers":[{"nameIdentifier":"468514","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyatake, Shin-Ichi"}],"nameIdentifiers":[{"nameIdentifier":"468515","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"近藤 夏子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468516","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平川 博一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468517","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"矢島 浩彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468518","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468519","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小野 公二","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"468520","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"High linear-energy-transfer radiation can overcome radioresistance of glioma stem-like cells to low linear-energy-transfer radiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"High linear-energy-transfer radiation can overcome radioresistance of glioma stem-like cells to low linear-energy-transfer radiation"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-11-17"},"publish_date":"2014-11-17","publish_status":"0","recid":"46948","relation_version_is_last":true,"title":["High linear-energy-transfer radiation can overcome radioresistance of glioma stem-like cells to low linear-energy-transfer radiation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:45:42.586170+00:00"}