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The developing central nervous system (CNS) is a highly radiosensitive tissue; however, the biological effects of heavy-ion radiation, which are greater than those of low-linear energy transfer (LET) radiation, are not well studied, especially in vivo in intact organisms. Here, we examined the effects of iron-ions on the developing CNS using vertebrate organism, fish embryos of medaka (Oryzias latipes).\nMaterials and methods: Medaka embryos at developmental stage 28 were irradiated with iron-ions at various doses of 0\u0026#8211;1.5 Gy. At 24 h after irradiation, radiation-induced apoptosis was examined using an acridine orange (AO) assay and histologically. To estimate the relative biological effectiveness (RBE), we quantified only characteristic AO-stained rosette-shaped apoptosis in the developing optic tectum (OT). 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However, all of the irradiated embryos hatched normally without apparent histological abnormalities in their brains.\nConclusion: Our present study indicates that the medaka embryo is a useful model for evaluating neurocytotoxic effects on the developing CNS induced by exposure to heavy iron-ions relevant to the aerospace radiation environment.", "subitem_description_type": "Abstract"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.3109/09553002.2011.584944", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0955-3002", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yasuda, Takako"}], "nameIdentifiers": [{"nameIdentifier": "462247", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oda, Syoji"}], "nameIdentifiers": [{"nameIdentifier": "462248", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yasuda, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "462249", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hibi, Yusuke"}], "nameIdentifiers": [{"nameIdentifier": "462250", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Anzai, Kazunori"}], "nameIdentifiers": [{"nameIdentifier": "462251", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitani, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "462252", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "保田 浩志", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "462253", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "安西 和紀", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "462254", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Neurocytotoxic effects of iron-ions on the developing brain measured in vivo using medaka (Oryzias latipes), a vertebrate model", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Neurocytotoxic effects of iron-ions on the developing brain measured in vivo using medaka (Oryzias latipes), a vertebrate model"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/46403", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-10-01"}, "publish_date": "2012-10-01", "publish_status": "0", "recid": "46403", "relation": {}, "relation_version_is_last": true, "title": ["Neurocytotoxic effects of iron-ions on the developing brain measured in vivo using medaka (Oryzias latipes), a vertebrate model"], "weko_shared_id": -1}
Neurocytotoxic effects of iron-ions on the developing brain measured in vivo using medaka (Oryzias latipes), a vertebrate model
https://repo.qst.go.jp/records/46403
https://repo.qst.go.jp/records/4640312bb0d11-7e2a-48ca-85a6-e89a182f03a8
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-10-01 | |||||
タイトル | ||||||
タイトル | Neurocytotoxic effects of iron-ions on the developing brain measured in vivo using medaka (Oryzias latipes), a vertebrate model | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Yasuda, Takako
× Yasuda, Takako× Oda, Syoji× Yasuda, Hiroshi× Hibi, Yusuke× Anzai, Kazunori× Mitani, Hiroshi× 保田 浩志× 安西 和紀 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Purpose: Exposure to heavy-ion radiation is considered a critical health risk on long-term space missions. The developing central nervous system (CNS) is a highly radiosensitive tissue; however, the biological effects of heavy-ion radiation, which are greater than those of low-linear energy transfer (LET) radiation, are not well studied, especially in vivo in intact organisms. Here, we examined the effects of iron-ions on the developing CNS using vertebrate organism, fish embryos of medaka (Oryzias latipes). Materials and methods: Medaka embryos at developmental stage 28 were irradiated with iron-ions at various doses of 0–1.5 Gy. At 24 h after irradiation, radiation-induced apoptosis was examined using an acridine orange (AO) assay and histologically. To estimate the relative biological effectiveness (RBE), we quantified only characteristic AO-stained rosette-shaped apoptosis in the developing optic tectum (OT). At the time of hatching, morphological abnormalities in the irradiated brain were examined histologically. Results: The dose–response curve utilizing an apoptotic index for the iron-ion irradiated embryos was much steeper than that for X-ray irradiated embryos, with RBE values of 3.7–4.2. Histological examinations of irradiated medaka brain at 24 h after irradiation showed AO-positive rosette-shaped clusters as aggregates of condensed nuclei, exhibiting a circular hole, mainly in the marginal area of the OT and in the retina. However, all of the irradiated embryos hatched normally without apparent histological abnormalities in their brains. Conclusion: Our present study indicates that the medaka embryo is a useful model for evaluating neurocytotoxic effects on the developing CNS induced by exposure to heavy iron-ions relevant to the aerospace radiation environment. |
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書誌情報 |
International Journal of Radiation Biology 巻 87, 号 9, p. 915-922, 発行日 2011-09 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0955-3002 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3109/09553002.2011.584944 |