{"created":"2023-05-15T14:36:03.230640+00:00","id":46403,"links":{},"metadata":{"_buckets":{"deposit":"42728042-c578-4405-b1a1-7c0fe45c2e48"},"_deposit":{"created_by":1,"id":"46403","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46403"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046403","sets":["1"]},"author_link":["462253","462252","462254","462247","462251","462248","462249","462250"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"922","bibliographicPageStart":"915","bibliographicVolumeNumber":"87","bibliographic_titles":[{"bibliographic_title":"International Journal of Radiation Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: Exposure to heavy-ion radiation is considered a critical health risk on long-term space missions. The developing central nervous system (CNS) is a highly radiosensitive tissue; however, the biological effects of heavy-ion radiation, which are greater than those of low-linear energy transfer (LET) radiation, are not well studied, especially in vivo in intact organisms. Here, we examined the effects of iron-ions on the developing CNS using vertebrate organism, fish embryos of medaka (Oryzias latipes).\nMaterials and methods: Medaka embryos at developmental stage 28 were irradiated with iron-ions at various doses of 0–1.5 Gy. At 24 h after irradiation, radiation-induced apoptosis was examined using an acridine orange (AO) assay and histologically. To estimate the relative biological effectiveness (RBE), we quantified only characteristic AO-stained rosette-shaped apoptosis in the developing optic tectum (OT). At the time of hatching, morphological abnormalities in the irradiated brain were examined histologically.\nResults: The dose–response curve utilizing an apoptotic index for the iron-ion irradiated embryos was much steeper than that for X-ray irradiated embryos, with RBE values of 3.7–4.2. Histological examinations of irradiated medaka brain at 24 h after irradiation showed AO-positive rosette-shaped clusters as aggregates of condensed nuclei, exhibiting a circular hole, mainly in the marginal area of the OT and in the retina. However, all of the irradiated embryos hatched normally without apparent histological abnormalities in their brains.\nConclusion: Our present study indicates that the medaka embryo is a useful model for evaluating neurocytotoxic effects on the developing CNS induced by exposure to heavy iron-ions relevant to the aerospace radiation environment.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3109/09553002.2011.584944","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0955-3002","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yasuda, Takako"}],"nameIdentifiers":[{"nameIdentifier":"462247","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oda, Syoji"}],"nameIdentifiers":[{"nameIdentifier":"462248","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yasuda, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"462249","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hibi, Yusuke"}],"nameIdentifiers":[{"nameIdentifier":"462250","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Anzai, Kazunori"}],"nameIdentifiers":[{"nameIdentifier":"462251","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mitani, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"462252","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"保田 浩志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462253","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安西 和紀","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462254","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Neurocytotoxic effects of iron-ions on the developing brain measured in vivo using medaka (Oryzias latipes), a vertebrate model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Neurocytotoxic effects of iron-ions on the developing brain measured in vivo using medaka (Oryzias latipes), a vertebrate model"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-10-01"},"publish_date":"2012-10-01","publish_status":"0","recid":"46403","relation_version_is_last":true,"title":["Neurocytotoxic effects of iron-ions on the developing brain measured in vivo using medaka (Oryzias latipes), a vertebrate model"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:52:14.845889+00:00"}