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Direct detection of p53-/- thymocyte appearing at an early stage of radiation-induced thymic lymphomagenesis in p53+/- heterozygous B10 mice
https://repo.qst.go.jp/records/45277
https://repo.qst.go.jp/records/4527720f64e0b-fba2-4f09-94ff-6184c58ea8a5
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-09-29 | |||||
| タイトル | ||||||
| タイトル | Direct detection of p53-/- thymocyte appearing at an early stage of radiation-induced thymic lymphomagenesis in p53+/- heterozygous B10 mice | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Aizawa, Shirou
× Aizawa, Shirou× Tanaka, Kaoru× Mori, Masahiko× Tsuji, Satsuki× Yoshida, Kazuko× 相澤 志郎× 田中 薫× 森 雅彦× 辻 さつき× 吉田 和子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: The appearance of tumor suppressor protein 53 (p53) -/- thymocytes at an early stage of radiation-induced lymphomagenesis was investigated in the p53 heterozygous (+/-) B10 mice following a single dose of irradiation, since most thymic lymphomas manifested the loss of the wild-type p53 allele and the loss of heterozygosity was thought to be an early event critical for radiation-induced thymic lymphomagenesis in p53+/- mice. \nMaterials and methods: The mice were exposed to a single dose (6 Gy) of irradiation to induce thymic lymphomas and, at various times after irradiation, treated with an extremely high dose (30 Gy) of whole-body irradiation to enrich p53-/- thymocytes and, 24 h later, the remaining thymocytes were assayed for cell surface markers and p53 genotype. \nResults: In a significant fraction of the p53+/- mice 5 weeks after 6 Gy irradiation, there was a relative increase in the number of cluster of differentiation (CD) 4+CD8+ thymocyte subpopulation among thymocytes remaining after 30 Gy irradiation. The CD4+CD8+ double-positive (DP) thymocytes were shown to contain p53-/- cells, and the number of p53-/- thymocytes was more than 105 in those individuals. \nConclusions: The results clearly indicated that an extremely high dose (30 Gy) of whole-body irradiation enabled us to directly detect p53-/- thymocytes in an abundant p53+/- thymocyte population and that proliferative p53-/- thymocytes develop in a CD4+CD8+ DP thymocyte subpopulation within a few weeks after a single dose (6 Gy) of irradiation. |
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| 書誌情報 |
International Journal of Radiation Biology 巻 83, 号 4, p. 259-267, 発行日 2007-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0955-3002 | |||||
