@article{oai:repo.qst.go.jp:00045277,
 author = {Aizawa, Shirou and Tanaka, Kaoru and Mori, Masahiko and Tsuji, Satsuki and Yoshida, Kazuko and 相澤 志郎 and 田中 薫 and 森 雅彦 and 辻 さつき and 吉田 和子},
 issue = {4},
 journal = {International Journal of Radiation Biology},
 month = {Apr},
 note = {Purpose: The appearance of tumor suppressor protein 53 (p53) -/- thymocytes at an early stage of radiation-induced lymphomagenesis was investigated in the p53 heterozygous (+/-) B10 mice following a single dose of irradiation, since most thymic lymphomas manifested the loss of the wild-type p53 allele and the loss of heterozygosity was thought to be an early event critical for radiation-induced thymic lymphomagenesis in p53+/- mice. 
\nMaterials and methods: The mice were exposed to a single dose (6 Gy) of irradiation to induce thymic lymphomas and, at various times after irradiation, treated with an extremely high dose (30 Gy) of whole-body irradiation to enrich p53-/- thymocytes and, 24 h later, the remaining thymocytes were assayed for cell surface markers and p53 genotype. 
\nResults: In a significant fraction of the p53+/- mice 5 weeks after 6 Gy irradiation, there was a relative increase in the number of cluster of differentiation (CD) 4+CD8+ thymocyte subpopulation among thymocytes remaining after 30 Gy irradiation. The CD4+CD8+ double-positive (DP) thymocytes were shown to contain p53-/- cells, and the number of p53-/- thymocytes was more than 105 in those individuals. 
\nConclusions: The results clearly indicated that an extremely high dose (30 Gy) of whole-body irradiation enabled us to directly detect p53-/- thymocytes in an abundant p53+/- thymocyte population and that proliferative p53-/- thymocytes develop in a CD4+CD8+ DP thymocyte subpopulation within a few weeks after a single dose (6 Gy) of irradiation.},
 pages = {259--267},
 title = {Direct detection of p53-/- thymocyte appearing at an early stage of radiation-induced thymic lymphomagenesis in p53+/- heterozygous B10 mice},
 volume = {83},
 year = {2007}
}