WEKO3
アイテム
{"_buckets": {"deposit": "c2ebfbd0-4969-4bf7-98ea-c0ad37e3c95b"}, "_deposit": {"created_by": 1, "id": "45228", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "45228"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00045228", "sets": ["1"]}, "author_link": ["449245", "449244", "449243", "449253", "449251", "449252", "449248", "449250", "449249", "449247", "449242", "449246"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-07", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "424", "bibliographicPageStart": "417", "bibliographicVolumeNumber": "49", "bibliographic_titles": [{"bibliographic_title": "Journal of Radiation Research"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "To evaluate whether the continuous treatment of two cytokine combinations is effective in megakaryocytopoiesis and thrombopoiesis in hematopoietic stem/progenitor cells exposed to heavy ion beams, the effects of a 2-step culture by a combination of recombinant human interleukin-3 (IL-3) + stem cell factor (SCF) + thrombopoietin (TPO), which just slightly protected against carbon-ion beam-induced damages, and a combination of IL-3 + TPO, which selectively stimulated the differentiation of the hematopoietic stem/progenitor cells to megakaryocytes and platelets, were examined. CD34+-hematopoietic stem/progenitor cells isolated from the human placental and umbilical cord blood were exposed to carbon-ion beams (LET = 50 keV/um) at 2 Gy. These cells were cultured under three cytokine conditions. The number of megakaryocytes, platelets and hematopoietic progenitors were assessed using a flow cytometer and a clonogenic assay at 14 and 21 days after irradiation, respectively. However, the efficacy of each 2-step culture was equal or lower than that of using the IL-3 + SCF + TPO combination alone and the 2-step culture could not induce megakaryocytes and platelets from hematopoietic stem/progenitor cells exposed to high LET-radiation such as carbon-ion beams. Therefore, additional cytokines and/or hematopoietic promoting compounds might be required to overcome damage to hematopoietic cells by high LET radiation.", "subitem_description_type": "Abstract"}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0449-3060", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Takahashi, Kenji"}], "nameIdentifiers": [{"nameIdentifier": "449242", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Monzen, Satoru"}], "nameIdentifiers": [{"nameIdentifier": "449243", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshino, Hironori"}], "nameIdentifiers": [{"nameIdentifier": "449244", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Abe, Yoshinao"}], "nameIdentifiers": [{"nameIdentifier": "449245", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Eguchi-Kasai, Kiyomi"}], "nameIdentifiers": [{"nameIdentifier": "449246", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kashiwakura, Ikuo"}], "nameIdentifiers": [{"nameIdentifier": "449247", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "高橋 賢次", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "449248", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "門前 暁", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "449249", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "吉野 浩教", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "449250", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "阿部 由直", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "449251", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "笠井 清美", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "449252", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "柏倉 幾郎", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "449253", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Effects of a 2-step culture with cytokine combinations on megakaryocytopoiesis and thrombopoiesis from carbon-ion beam-irradiated human hematopoietic stem/progenitor cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Effects of a 2-step culture with cytokine combinations on megakaryocytopoiesis and thrombopoiesis from carbon-ion beam-irradiated human hematopoietic stem/progenitor cells"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/45228", "pubdate": {"attribute_name": "公開日", "attribute_value": "2008-07-23"}, "publish_date": "2008-07-23", "publish_status": "0", "recid": "45228", "relation": {}, "relation_version_is_last": true, "title": ["Effects of a 2-step culture with cytokine combinations on megakaryocytopoiesis and thrombopoiesis from carbon-ion beam-irradiated human hematopoietic stem/progenitor cells"], "weko_shared_id": -1}
Effects of a 2-step culture with cytokine combinations on megakaryocytopoiesis and thrombopoiesis from carbon-ion beam-irradiated human hematopoietic stem/progenitor cells
https://repo.qst.go.jp/records/45228
https://repo.qst.go.jp/records/45228e7a81ee9-b385-4fe5-967f-df74ece67a68
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2008-07-23 | |||||
タイトル | ||||||
タイトル | Effects of a 2-step culture with cytokine combinations on megakaryocytopoiesis and thrombopoiesis from carbon-ion beam-irradiated human hematopoietic stem/progenitor cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Takahashi, Kenji
× Takahashi, Kenji× Monzen, Satoru× Yoshino, Hironori× Abe, Yoshinao× Eguchi-Kasai, Kiyomi× Kashiwakura, Ikuo× 高橋 賢次× 門前 暁× 吉野 浩教× 阿部 由直× 笠井 清美× 柏倉 幾郎 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To evaluate whether the continuous treatment of two cytokine combinations is effective in megakaryocytopoiesis and thrombopoiesis in hematopoietic stem/progenitor cells exposed to heavy ion beams, the effects of a 2-step culture by a combination of recombinant human interleukin-3 (IL-3) + stem cell factor (SCF) + thrombopoietin (TPO), which just slightly protected against carbon-ion beam-induced damages, and a combination of IL-3 + TPO, which selectively stimulated the differentiation of the hematopoietic stem/progenitor cells to megakaryocytes and platelets, were examined. CD34+-hematopoietic stem/progenitor cells isolated from the human placental and umbilical cord blood were exposed to carbon-ion beams (LET = 50 keV/um) at 2 Gy. These cells were cultured under three cytokine conditions. The number of megakaryocytes, platelets and hematopoietic progenitors were assessed using a flow cytometer and a clonogenic assay at 14 and 21 days after irradiation, respectively. However, the efficacy of each 2-step culture was equal or lower than that of using the IL-3 + SCF + TPO combination alone and the 2-step culture could not induce megakaryocytes and platelets from hematopoietic stem/progenitor cells exposed to high LET-radiation such as carbon-ion beams. Therefore, additional cytokines and/or hematopoietic promoting compounds might be required to overcome damage to hematopoietic cells by high LET radiation. | |||||
書誌情報 |
Journal of Radiation Research 巻 49, 号 4, p. 417-424, 発行日 2008-07 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0449-3060 |