{"created":"2023-05-15T14:35:01.186212+00:00","id":45228,"links":{},"metadata":{"_buckets":{"deposit":"c2ebfbd0-4969-4bf7-98ea-c0ad37e3c95b"},"_deposit":{"created_by":1,"id":"45228","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45228"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045228","sets":["1"]},"author_link":["449245","449244","449243","449253","449251","449252","449248","449250","449249","449247","449242","449246"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"424","bibliographicPageStart":"417","bibliographicVolumeNumber":"49","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To evaluate whether the continuous treatment of two cytokine combinations is effective in megakaryocytopoiesis and thrombopoiesis in hematopoietic stem/progenitor cells exposed to heavy ion beams, the effects of a 2-step culture by a combination of recombinant human interleukin-3 (IL-3) + stem cell factor (SCF) + thrombopoietin (TPO), which just slightly protected against carbon-ion beam-induced damages, and a combination of IL-3 + TPO, which selectively stimulated the differentiation of the hematopoietic stem/progenitor cells to megakaryocytes and platelets, were examined. CD34+-hematopoietic stem/progenitor cells isolated from the human placental and umbilical cord blood were exposed to carbon-ion beams (LET = 50 keV/um) at 2 Gy. These cells were cultured under three cytokine conditions. The number of megakaryocytes, platelets and hematopoietic progenitors were assessed using a flow cytometer and a clonogenic assay at 14 and 21 days after irradiation, respectively. However, the efficacy of each 2-step culture was equal or lower than that of using the IL-3 + SCF + TPO combination alone and the 2-step culture could not induce megakaryocytes and platelets from hematopoietic stem/progenitor cells exposed to high LET-radiation such as carbon-ion beams. Therefore, additional cytokines and/or hematopoietic promoting compounds might be required to overcome damage to hematopoietic cells by high LET radiation.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0449-3060","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahashi, Kenji"}],"nameIdentifiers":[{"nameIdentifier":"449242","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Monzen, Satoru"}],"nameIdentifiers":[{"nameIdentifier":"449243","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshino, Hironori"}],"nameIdentifiers":[{"nameIdentifier":"449244","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Abe, Yoshinao"}],"nameIdentifiers":[{"nameIdentifier":"449245","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Eguchi-Kasai, Kiyomi"}],"nameIdentifiers":[{"nameIdentifier":"449246","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kashiwakura, Ikuo"}],"nameIdentifiers":[{"nameIdentifier":"449247","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 賢次","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449248","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"門前 暁","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449249","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉野 浩教","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449250","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"阿部 由直","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449251","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"笠井 清美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449252","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柏倉 幾郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"449253","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of a 2-step culture with cytokine combinations on megakaryocytopoiesis and thrombopoiesis from carbon-ion beam-irradiated human hematopoietic stem/progenitor cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of a 2-step culture with cytokine combinations on megakaryocytopoiesis and thrombopoiesis from carbon-ion beam-irradiated human hematopoietic stem/progenitor cells"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-07-23"},"publish_date":"2008-07-23","publish_status":"0","recid":"45228","relation_version_is_last":true,"title":["Effects of a 2-step culture with cytokine combinations on megakaryocytopoiesis and thrombopoiesis from carbon-ion beam-irradiated human hematopoietic stem/progenitor cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:06:13.287086+00:00"}