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We investigated the therapeutic effect of Auger electrons emitted by 111In-labeled phosphorothioate antisense oligonucleotides on human neuroblastoma cells in which N-myc was overexpressed. METHODS: Human SK-N-DZ neuroblastoma cells (5 x 10(6) cells) were treated with cationic reverse-phase evaporation vesicles (REVs) encapsulating 111In-labeled antisense (40 MBq/2 nmol of oligonucleotides/mumol of total phospholipids) that had an average diameter of 250 nm. Hybridization of the radiolabeled oligonucleotides with N-myc messenger RNA (mRNA), N-myc expression, and cell proliferation were investigated. The tumorigenicity of treated cells was analyzed in nude mice. Nonradiolabeled antisense, 111In-labeled sense, or empty cationic REVs were used as controls. RESULTS: 111In-Labeled antisense, which hybridized with N-myc mRNA, was detected in cells at 12 and 24 h after the initiation of treatment. Reduced N-myc expression and inhibited cell proliferation were shown in the same cells at 48 h after the completion of treatment. N-myc expression-suppressed cells produced intraperitoneal tumors in nude mice, but the average weight of the tumors was lower than that of tumors in control mice. CONCLUSION: Auger electrons emitted from 111In in close proximity to their target N-myc mRNA may prolong the time to cell proliferation in human neuroblastoma cells due to inhibition of the translation of N-myc. Auger electron therapy therefore has potential as an internally delivered molecular radiotherapy targeting the mRNA of a tumor cell.", "subitem_description_type": "Abstract"}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0161-5505", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Watanabe, Naoyuki"}], "nameIdentifiers": [{"nameIdentifier": "444869", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanada, Shuji"}], "nameIdentifiers": [{"nameIdentifier": "444870", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yonekura, Yoshiharu"}], "nameIdentifiers": [{"nameIdentifier": "444871", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sasaki, Yasuhito"}], "nameIdentifiers": [{"nameIdentifier": "444872", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "et.al"}], "nameIdentifiers": [{"nameIdentifier": "444873", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "渡辺 直行", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "444874", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "棚田 修二", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "444875", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "米倉 義晴", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "444876", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "佐々木 康人", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "444877", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Molecular Therapy of Human Neuroblastoma Cells Using Auger Electrons of 111In-Labeled N-myc Antisense Oligonucleotides", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Molecular Therapy of Human Neuroblastoma Cells Using Auger Electrons of 111In-Labeled N-myc Antisense Oligonucleotides"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/44802", "pubdate": {"attribute_name": "公開日", "attribute_value": "2007-03-08"}, "publish_date": "2007-03-08", "publish_status": "0", "recid": "44802", "relation": {}, "relation_version_is_last": true, "title": ["Molecular Therapy of Human Neuroblastoma Cells Using Auger Electrons of 111In-Labeled N-myc Antisense Oligonucleotides"], "weko_shared_id": -1}
Molecular Therapy of Human Neuroblastoma Cells Using Auger Electrons of 111In-Labeled N-myc Antisense Oligonucleotides
https://repo.qst.go.jp/records/44802
https://repo.qst.go.jp/records/44802738ee92b-60be-4a91-b853-f3e43858a17c
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-03-08 | |||||
タイトル | ||||||
タイトル | Molecular Therapy of Human Neuroblastoma Cells Using Auger Electrons of 111In-Labeled N-myc Antisense Oligonucleotides | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Watanabe, Naoyuki
× Watanabe, Naoyuki× Tanada, Shuji× Yonekura, Yoshiharu× Sasaki, Yasuhito× et.al× 渡辺 直行× 棚田 修二× 米倉 義晴× 佐々木 康人 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Auger electrons can create breaks in nucleic acids, giving them possible therapeutic utility. We investigated the therapeutic effect of Auger electrons emitted by 111In-labeled phosphorothioate antisense oligonucleotides on human neuroblastoma cells in which N-myc was overexpressed. METHODS: Human SK-N-DZ neuroblastoma cells (5 x 10(6) cells) were treated with cationic reverse-phase evaporation vesicles (REVs) encapsulating 111In-labeled antisense (40 MBq/2 nmol of oligonucleotides/mumol of total phospholipids) that had an average diameter of 250 nm. Hybridization of the radiolabeled oligonucleotides with N-myc messenger RNA (mRNA), N-myc expression, and cell proliferation were investigated. The tumorigenicity of treated cells was analyzed in nude mice. Nonradiolabeled antisense, 111In-labeled sense, or empty cationic REVs were used as controls. RESULTS: 111In-Labeled antisense, which hybridized with N-myc mRNA, was detected in cells at 12 and 24 h after the initiation of treatment. Reduced N-myc expression and inhibited cell proliferation were shown in the same cells at 48 h after the completion of treatment. N-myc expression-suppressed cells produced intraperitoneal tumors in nude mice, but the average weight of the tumors was lower than that of tumors in control mice. CONCLUSION: Auger electrons emitted from 111In in close proximity to their target N-myc mRNA may prolong the time to cell proliferation in human neuroblastoma cells due to inhibition of the translation of N-myc. Auger electron therapy therefore has potential as an internally delivered molecular radiotherapy targeting the mRNA of a tumor cell. | |||||
書誌情報 |
Journal of Nuclear Medicine 巻 47, 号 10, p. 1670-1677, 発行日 2006-10 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0161-5505 |