{"created":"2023-05-15T14:34:40.025685+00:00","id":44802,"links":{},"metadata":{"_buckets":{"deposit":"ea997f47-b3c5-4af2-805d-11a011bf4403"},"_deposit":{"created_by":1,"id":"44802","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44802"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044802","sets":["1"]},"author_link":["444870","444869","444872","444874","444871","444877","444873","444876","444875"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"1677","bibliographicPageStart":"1670","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"Journal of Nuclear Medicine"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Auger electrons can create breaks in nucleic acids, giving them possible therapeutic utility. We investigated the therapeutic effect of Auger electrons emitted by 111In-labeled phosphorothioate antisense oligonucleotides on human neuroblastoma cells in which N-myc was overexpressed. METHODS: Human SK-N-DZ neuroblastoma cells (5 x 10(6) cells) were treated with cationic reverse-phase evaporation vesicles (REVs) encapsulating 111In-labeled antisense (40 MBq/2 nmol of oligonucleotides/mumol of total phospholipids) that had an average diameter of 250 nm. Hybridization of the radiolabeled oligonucleotides with N-myc messenger RNA (mRNA), N-myc expression, and cell proliferation were investigated. The tumorigenicity of treated cells was analyzed in nude mice. Nonradiolabeled antisense, 111In-labeled sense, or empty cationic REVs were used as controls. RESULTS: 111In-Labeled antisense, which hybridized with N-myc mRNA, was detected in cells at 12 and 24 h after the initiation of treatment. Reduced N-myc expression and inhibited cell proliferation were shown in the same cells at 48 h after the completion of treatment. N-myc expression-suppressed cells produced intraperitoneal tumors in nude mice, but the average weight of the tumors was lower than that of tumors in control mice. CONCLUSION: Auger electrons emitted from 111In in close proximity to their target N-myc mRNA may prolong the time to cell proliferation in human neuroblastoma cells due to inhibition of the translation of N-myc. Auger electron therapy therefore has potential as an internally delivered molecular radiotherapy targeting the mRNA of a tumor cell.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0161-5505","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Watanabe, Naoyuki"}],"nameIdentifiers":[{"nameIdentifier":"444869","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanada, Shuji"}],"nameIdentifiers":[{"nameIdentifier":"444870","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yonekura, Yoshiharu"}],"nameIdentifiers":[{"nameIdentifier":"444871","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasaki, Yasuhito"}],"nameIdentifiers":[{"nameIdentifier":"444872","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"444873","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"渡辺 直行","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"444874","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"棚田 修二","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"444875","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"米倉 義晴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"444876","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐々木 康人","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"444877","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Molecular Therapy of Human Neuroblastoma Cells Using Auger Electrons of 111In-Labeled N-myc Antisense Oligonucleotides","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Molecular Therapy of Human Neuroblastoma Cells Using Auger Electrons of 111In-Labeled N-myc Antisense Oligonucleotides"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-03-08"},"publish_date":"2007-03-08","publish_status":"0","recid":"44802","relation_version_is_last":true,"title":["Molecular Therapy of Human Neuroblastoma Cells Using Auger Electrons of 111In-Labeled N-myc Antisense Oligonucleotides"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:11:10.878571+00:00"}