量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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The dose in the plateau region of carbon-ion (C-ion) beams before the Bragg peak is not necessarily zero. Thus, the effects of the plateau C-ion beams on biomolecules is considerable importance to eliminate the adverse effects of radiation therapy on normal tissues adjacent to the tumor. We have recently demonstrated that isopropyl myristate (IPM), one of lipid molecules, is more susceptible to the plateau C-ion beams than to X-rays using 2,2-diphenyl-1-picrylhydrazyl radical used as a redox probe (Nakanishi I, et al. Org Biomol Chem 2018;16:1272). In this study, the comparison of the effect of the plateau C-ion beams with that of X-rays on lipid molecules was investigated by 2,2,6-trimethyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)-6-pentylpiperidine-1-oxyl (NBD-Pen), a highly selective fluorescence probe for lipid radicals with high sensitivity (Yamada K, et al. Nat Chem Biol 2016;12:608). NBD-Pen reacts with the lipid radicals generated by the oxidation of lipid molecules to form highly fluorescent radical adducts. IPM solutions of NBD-Pen (5.0 µM) were irradiated by the plateau C-ion beams (290 MeV, 13 keV/µm) using the Heavy Ion Medical Accelerator in Chiba (HIMAC) at room temperature. An increase in fluorescence intensity at 509 nm was observed for the IPM solutions of NBD-Pen after the C-ion irradiation (5, 10, 15, 20, and 30 Gy) on a Shimadzu RF-5300PC spectrofluorophotometer. This indicates that radical species were generated by the C-ion irradiation to IPM. The effect of the plateau C-ion beams on the lipid oxidation will be discussed as compared to that of X-rays based on the results obtained in this study.
放射線照射で脂質分子から生成するラジカル種の 蛍光プローブによる検出
