@misc{oai:repo.qst.go.jp:00077459,
 author = {中西, 郁夫 and 荘司, 好美 and 上野, 恵美 and 関根(鈴木), 絵美子 and 松岡, 悠太 and 下川, 卓志 and 山田, 健一 and 松本, 謙一郎 and Nakanishi, Ikuo and Shoji, Yoshimi and Ueno, Megumi and Sekine, Emiko and Shimokawa, Takashi and Matsumoto, Kenichiro},
 month = {Nov},
 note = {The dose in the plateau region of carbon-ion (C-ion) beams before the Bragg peak is not necessarily zero.  Thus, the effects of the plateau C-ion beams on biomolecules is considerable importance to eliminate the adverse effects of radiation therapy on normal tissues adjacent to the tumor.  We have recently demonstrated that isopropyl myristate (IPM), one of lipid molecules, is more susceptible to the plateau C-ion beams than to X-rays using 2,2-diphenyl-1-picrylhydrazyl radical used as a redox probe (Nakanishi I, et al. Org Biomol Chem 2018;16:1272).  In this study, the comparison of the effect of the plateau C-ion beams with that of X-rays on lipid molecules was investigated by 2,2,6-trimethyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)-6-pentylpiperidine-1-oxyl (NBD-Pen), a highly selective fluorescence probe for lipid radicals with high sensitivity (Yamada K, et al. Nat Chem Biol 2016;12:608).  NBD-Pen reacts with the lipid radicals generated by the oxidation of lipid molecules to form highly fluorescent radical adducts.  IPM solutions of NBD-Pen (5.0 µM) were irradiated by the plateau C-ion beams (290 MeV, 13 keV/µm) using the Heavy Ion Medical Accelerator in Chiba (HIMAC) at room temperature.  An increase in fluorescence intensity at 509 nm was observed for the IPM solutions of NBD-Pen after the C-ion irradiation (5, 10, 15, 20, and 30 Gy) on a Shimadzu RF-5300PC spectrofluorophotometer.  This indicates that radical species were generated by the C-ion irradiation to IPM.  The effect of the plateau C-ion beams on the lipid oxidation will be discussed as compared to that of X-rays based on the results obtained in this study., 日本放射線影響学会第62回大会},
 title = {放射線照射で脂質分子から生成するラジカル種の 蛍光プローブによる検出},
 year = {2019}
}