量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
Thank you very much for using our website. On the 11th of March 2019, this site was moved from our own network server to the JAIRO Cloud network server. If you previously bookmarked this site, that bookmark will no longer work. We would be grateful if you could bookmark the website again. Thank you very much for your understanding and cooperation.
While Deinococcus radiodurans, the type species of the genus Deinococcus, does not possess any genes involved in translesion DNA synthesis, the draft genome sequencing of Deinococcus grandis KS0485 revealed that this bacterium possesses two lexA-imuB-dnaE2 gene clusters, which will be involved in translesion DNA synthesis. In this study, we expressed deinococcal dnaE2 and imuB genes in Escherichia coli to further delineate the function of these genes. The dnaE2 and imuB genes were cloned in pET-based vector and p15A-base vector, respectively, and introduced in E. coli BL21(DE3) cells. Mutant frequency was measured by colony formation assay based on rifampicin resistance. As a result, when both the dnaE2 and imuB genes were expressed in E. coli under IPTG induction, the mutant frequency was increased, confirming that the functionality of dnaE2 and imuB as mutagenesis genes. However, when dnaE2 or imuB gene was separately expressed in E. coli, no increase in mutant frequency was observed. These results suggest that both gene products are necessary to operate mutagenesis.
Expression of translesion DNA polymerase genes from Deinococcus grandis in Escherichia coli
