量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum Science and Technology.
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Relatively young (8-week-old) selenium deficient (SeD) mouse model was prepared using SeD diet. Selenium (Se), which is an essential trace element, has important roles in the biological redox regulation system as the catalytic center of glutathione peroxidases (GSH-Px).
Mice were fed on torula yeast based SeD diet (F2SeDD, Oriental Yeast Co., Ltd., Tokyo) and ultra-pure water (mili-Q water). Several different timings for starting SeD diet were demonstrated and weekly time course of liver GSH-Px activities after starting SeD diet were monitored.
Postweaning (4-week-old or older) start of SeD diet gives relatively older (> 8-week-old) SeD mice. The same procedure which was reported for a Se-deficient rat model (Matsumoto et al., Biol. Pharm. Bull. 23, 641-644, 2000.) could not prepared Se-deficient mouse model due to neonatal death. Starting SeD diet to the mother mouse from 2 weeks after giving birth could give 8-week-old SeD mouse model (SeD-trial-2w). Tissue GSH-Px activities of 8-week-old SeD-trial-2w group were almost non, while the normal group (fed on normal diet, MB-1) and SeC group (fed on SeD diet + 1.5 mg Se in drinking water) could keep GSH-Px activity.
This SeD-trial-2w mouse model can be proposed for an H2O2-induced oxidative stress model for future experiments.
会議概要（会議名, 開催地, 会期, 主催者等）
The 19th biennial meeting for the Society for Free Radical Research International (SFRRI2018)
Preparation of Selenium-Deficient Mouse Model and its Glutathione Peroxidase Activity
