WEKO3
アイテム
{"_buckets": {"deposit": "315fd2ec-5ca7-4eca-bb44-bcd42d88b3a9"}, "_deposit": {"created_by": 1, "id": "46771", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "46771"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00046771", "sets": ["1"]}, "author_link": ["466465", "466466", "466474", "466476", "466473", "466468", "466471", "466475", "466470", "466472", "466469", "466467", "466464"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-2", "bibliographicPageEnd": "107", "bibliographicPageStart": "101", "bibliographicVolumeNumber": "756", "bibliographic_titles": [{"bibliographic_title": "Mutation Research"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We have studied the induction of chromosome aberrations in human fibroblasts exposed in G0/G1 to X-rays or heavy ions to study the influence of G1 cell cycle arrest. Confluent normal fibroblasts were exposed to X-rays or accelerated particles with different LET values and chromosome aberrations were investigated in the first G0/G1 and G2//M phase. The particles used here were 490MeV/nucleon Si, 500MeV/nucleon Fe, and 200MeV/nucleon Fe ions. Cells were subcultured 24h after exposure and premature chromosome condensation (PCC) was performed by fusion-induced method for analysis of G0/G1 cells, and chemically-induced method for analysis of G2 and metaphase cells. Chromosome damage was assessed in chromosomes 1 and 3 using whole chromosome fluorescence in situ hybridization (FISH). Cell cycle was analyzed by flow cytometry at different incubation times following subculture. After irradiation with 2Gy of high-LET particles, the yields of chromosome aberrations and fragments were significantly higher in G0/G1 phase than in G2/M phase, whereas similar yields of damage were measured in both phases after exposure to X-rays. In contrast, the yield of misrepair, assessed by the number of color junctions, was similar in the G0/G1 and G2/M phases after exposure to either X-rays or high-LET particles. The yields of chromosome aberrations, fragments, and color junctions in both the G0/G1 and the G2/M phases, increased with LET up to 200keV/μm, then decreased for 440keV/μm Fe particles. A good correlation was found between chromosome aberrations in both G0/G1 and G2/M cells and survival fractions after 2Gy of different LET radiations, although the slopes were steeper for the G0/G1 cells. Flow cytometry analysis indicated that high-LET particles induce more non cycling G0/G1 cells within 48h of subculture than X-rays, suggesting that chromosome aberrations scored at the G2/M phase may not accurately describe the true radiation effect.", "subitem_description_type": "Abstract"}]}, "item_8_relation_13": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "23688614", "subitem_relation_type_select": "PMID"}}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.mrgentox.2013.05.005", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0027-5107", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "hua, Liu Cui"}], "nameIdentifiers": [{"nameIdentifier": "466464", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawata, Tetsuya"}], "nameIdentifiers": [{"nameIdentifier": "466465", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Furusawa, Yoshiya"}], "nameIdentifiers": [{"nameIdentifier": "466466", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Zhou, Guangming"}], "nameIdentifiers": [{"nameIdentifier": "466467", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Inoue, Kohei"}], "nameIdentifiers": [{"nameIdentifier": "466468", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fukada, Junichi"}], "nameIdentifiers": [{"nameIdentifier": "466469", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kota, Ryuichi"}], "nameIdentifiers": [{"nameIdentifier": "466470", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "George, Kerry"}], "nameIdentifiers": [{"nameIdentifier": "466471", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Cucinotta, Francis"}], "nameIdentifiers": [{"nameIdentifier": "466472", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okayasu, Ryuichi"}], "nameIdentifiers": [{"nameIdentifier": "466473", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "劉 翠華", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "466474", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "古澤 佳也", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "466475", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "岡安 隆一", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "466476", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET).", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET)."}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/46771", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-07-11"}, "publish_date": "2014-07-11", "publish_status": "0", "recid": "46771", "relation": {}, "relation_version_is_last": true, "title": ["Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET)."], "weko_shared_id": -1}
Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET).
https://repo.qst.go.jp/records/46771
https://repo.qst.go.jp/records/46771c6fed783-0f68-4c05-8c2e-4a25d5427020
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2014-07-11 | |||||
タイトル | ||||||
タイトル | Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET). | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
hua, Liu Cui
× hua, Liu Cui× Kawata, Tetsuya× Furusawa, Yoshiya× Zhou, Guangming× Inoue, Kohei× Fukada, Junichi× Kota, Ryuichi× George, Kerry× Cucinotta, Francis× Okayasu, Ryuichi× 劉 翠華× 古澤 佳也× 岡安 隆一 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have studied the induction of chromosome aberrations in human fibroblasts exposed in G0/G1 to X-rays or heavy ions to study the influence of G1 cell cycle arrest. Confluent normal fibroblasts were exposed to X-rays or accelerated particles with different LET values and chromosome aberrations were investigated in the first G0/G1 and G2//M phase. The particles used here were 490MeV/nucleon Si, 500MeV/nucleon Fe, and 200MeV/nucleon Fe ions. Cells were subcultured 24h after exposure and premature chromosome condensation (PCC) was performed by fusion-induced method for analysis of G0/G1 cells, and chemically-induced method for analysis of G2 and metaphase cells. Chromosome damage was assessed in chromosomes 1 and 3 using whole chromosome fluorescence in situ hybridization (FISH). Cell cycle was analyzed by flow cytometry at different incubation times following subculture. After irradiation with 2Gy of high-LET particles, the yields of chromosome aberrations and fragments were significantly higher in G0/G1 phase than in G2/M phase, whereas similar yields of damage were measured in both phases after exposure to X-rays. In contrast, the yield of misrepair, assessed by the number of color junctions, was similar in the G0/G1 and G2/M phases after exposure to either X-rays or high-LET particles. The yields of chromosome aberrations, fragments, and color junctions in both the G0/G1 and the G2/M phases, increased with LET up to 200keV/μm, then decreased for 440keV/μm Fe particles. A good correlation was found between chromosome aberrations in both G0/G1 and G2/M cells and survival fractions after 2Gy of different LET radiations, although the slopes were steeper for the G0/G1 cells. Flow cytometry analysis indicated that high-LET particles induce more non cycling G0/G1 cells within 48h of subculture than X-rays, suggesting that chromosome aberrations scored at the G2/M phase may not accurately describe the true radiation effect. | |||||
書誌情報 |
Mutation Research 巻 756, 号 1-2, p. 101-107, 発行日 2013-08 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0027-5107 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 23688614 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.mrgentox.2013.05.005 |