{"created":"2023-05-15T14:36:19.692588+00:00","id":46771,"links":{},"metadata":{"_buckets":{"deposit":"315fd2ec-5ca7-4eca-bb44-bcd42d88b3a9"},"_deposit":{"created_by":1,"id":"46771","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46771"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046771","sets":["1"]},"author_link":["466465","466466","466474","466476","466473","466468","466471","466475","466470","466472","466469","466467","466464"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"107","bibliographicPageStart":"101","bibliographicVolumeNumber":"756","bibliographic_titles":[{"bibliographic_title":"Mutation Research"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We have studied the induction of chromosome aberrations in human fibroblasts exposed in G0/G1 to X-rays or heavy ions to study the influence of G1 cell cycle arrest. Confluent normal fibroblasts were exposed to X-rays or accelerated particles with different LET values and chromosome aberrations were investigated in the first G0/G1 and G2//M phase. The particles used here were 490MeV/nucleon Si, 500MeV/nucleon Fe, and 200MeV/nucleon Fe ions. Cells were subcultured 24h after exposure and premature chromosome condensation (PCC) was performed by fusion-induced method for analysis of G0/G1 cells, and chemically-induced method for analysis of G2 and metaphase cells. Chromosome damage was assessed in chromosomes 1 and 3 using whole chromosome fluorescence in situ hybridization (FISH). Cell cycle was analyzed by flow cytometry at different incubation times following subculture. After irradiation with 2Gy of high-LET particles, the yields of chromosome aberrations and fragments were significantly higher in G0/G1 phase than in G2/M phase, whereas similar yields of damage were measured in both phases after exposure to X-rays. In contrast, the yield of misrepair, assessed by the number of color junctions, was similar in the G0/G1 and G2/M phases after exposure to either X-rays or high-LET particles. The yields of chromosome aberrations, fragments, and color junctions in both the G0/G1 and the G2/M phases, increased with LET up to 200keV/μm, then decreased for 440keV/μm Fe particles. A good correlation was found between chromosome aberrations in both G0/G1 and G2/M cells and survival fractions after 2Gy of different LET radiations, although the slopes were steeper for the G0/G1 cells. Flow cytometry analysis indicated that high-LET particles induce more non cycling G0/G1 cells within 48h of subculture than X-rays, suggesting that chromosome aberrations scored at the G2/M phase may not accurately describe the true radiation effect.","subitem_description_type":"Abstract"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"23688614","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.mrgentox.2013.05.005","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0027-5107","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"hua, Liu Cui"}],"nameIdentifiers":[{"nameIdentifier":"466464","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawata, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"466465","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furusawa, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"466466","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhou, Guangming"}],"nameIdentifiers":[{"nameIdentifier":"466467","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Inoue, Kohei"}],"nameIdentifiers":[{"nameIdentifier":"466468","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukada, Junichi"}],"nameIdentifiers":[{"nameIdentifier":"466469","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kota, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"466470","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"George, Kerry"}],"nameIdentifiers":[{"nameIdentifier":"466471","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Cucinotta, Francis"}],"nameIdentifiers":[{"nameIdentifier":"466472","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"466473","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"劉 翠華","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"466474","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤 佳也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"466475","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"466476","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET).","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET)."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-07-11"},"publish_date":"2014-07-11","publish_status":"0","recid":"46771","relation_version_is_last":true,"title":["Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET)."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:47:47.636105+00:00"}