WEKO3
アイテム
{"_buckets": {"deposit": "f16dcd46-5c43-40ef-b5e5-6d17afdfdff3"}, "_deposit": {"created_by": 1, "id": "44246", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "44246"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00044246", "sets": ["1"]}, "author_link": ["439613", "439611", "439612", "439610", "439614", "439609", "439607", "439608"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "37", "bibliographicPageEnd": "38714", "bibliographicPageStart": "38710", "bibliographicVolumeNumber": "279", "bibliographic_titles": [{"bibliographic_title": "The Journal of Biological Chemistry"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "1-Methyl-4-phenylpyridinium ion (MPP+), an active metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, induces cell death and inhibition of cell proliferation in various cells. However, the mechanism whereby MPP+ inhibits cell proliferation is still unclear. In this study, we found that MPP+ suppressed the proliferation with accumulation in G1 phase without inducing cell death in p53-deficient MG63 osteosarcoma cells. MPP+ induced hypophosphorylation of retinoblastoma protein and rapidly down-regulated the protein but not mRNA levels of cyclin D1 in MG63 cells. The down-regulation of cyclin D1 protein was suppressed by a proteasome inhibitor, MG132. The cyclin D1 down-regulation by MPP+ was also observed in p53-positive PC12, HeLa S3, and HeLa 0 cells, which are a subclone of HeLa S3 lacking mitochondrial DNA. Moreover, MPP+ dephosphorylated Akt in PC12 cells, which was rescued by the pretreatment with nerve growth factor. In addition, the pretreatment with nerve growth factor or lithium chloride, a glycogen synthase kinase-3 inhibitor, suppressed the cyclin D1 down-regulation caused by MPP+. Our results demonstrate that MPP+ induces cell cycle arrest independently of its mitochondrial toxicity or the p53 status of the target cells, but rather through the proteasome- and phosphatidylinositol 3-Akt-glycogen synthase kinase-3-dependent cyclin D1 degradation.", "subitem_description_type": "Abstract"}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0021-9258", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Jie, Bai"}], "nameIdentifiers": [{"nameIdentifier": "439607", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakamura, Hajime"}], "nameIdentifiers": [{"nameIdentifier": "439608", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ueda, Shugo"}], "nameIdentifiers": [{"nameIdentifier": "439609", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kwon, Yong-Won"}], "nameIdentifiers": [{"nameIdentifier": "439610", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanaka, Toru"}], "nameIdentifiers": [{"nameIdentifier": "439611", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ban, Sadayuki"}], "nameIdentifiers": [{"nameIdentifier": "439612", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yodoi, Junji"}], "nameIdentifiers": [{"nameIdentifier": "439613", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "伴 貞幸", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "439614", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Proteasome-dependent Degradation of Cyclin D1 in 1-Methyl-4-phenylpyridinium Ion (MPP+)-induced Cell Cycle Arrest", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Proteasome-dependent Degradation of Cyclin D1 in 1-Methyl-4-phenylpyridinium Ion (MPP+)-induced Cell Cycle Arrest"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/44246", "pubdate": {"attribute_name": "公開日", "attribute_value": "2006-06-13"}, "publish_date": "2006-06-13", "publish_status": "0", "recid": "44246", "relation": {}, "relation_version_is_last": true, "title": ["Proteasome-dependent Degradation of Cyclin D1 in 1-Methyl-4-phenylpyridinium Ion (MPP+)-induced Cell Cycle Arrest"], "weko_shared_id": -1}
Proteasome-dependent Degradation of Cyclin D1 in 1-Methyl-4-phenylpyridinium Ion (MPP+)-induced Cell Cycle Arrest
https://repo.qst.go.jp/records/44246
https://repo.qst.go.jp/records/44246f9410ea0-cb27-405a-ab70-0cc4b4e3f1dd
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2006-06-13 | |||||
タイトル | ||||||
タイトル | Proteasome-dependent Degradation of Cyclin D1 in 1-Methyl-4-phenylpyridinium Ion (MPP+)-induced Cell Cycle Arrest | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Jie, Bai
× Jie, Bai× Nakamura, Hajime× Ueda, Shugo× Kwon, Yong-Won× Tanaka, Toru× Ban, Sadayuki× Yodoi, Junji× 伴 貞幸 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 1-Methyl-4-phenylpyridinium ion (MPP+), an active metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, induces cell death and inhibition of cell proliferation in various cells. However, the mechanism whereby MPP+ inhibits cell proliferation is still unclear. In this study, we found that MPP+ suppressed the proliferation with accumulation in G1 phase without inducing cell death in p53-deficient MG63 osteosarcoma cells. MPP+ induced hypophosphorylation of retinoblastoma protein and rapidly down-regulated the protein but not mRNA levels of cyclin D1 in MG63 cells. The down-regulation of cyclin D1 protein was suppressed by a proteasome inhibitor, MG132. The cyclin D1 down-regulation by MPP+ was also observed in p53-positive PC12, HeLa S3, and HeLa 0 cells, which are a subclone of HeLa S3 lacking mitochondrial DNA. Moreover, MPP+ dephosphorylated Akt in PC12 cells, which was rescued by the pretreatment with nerve growth factor. In addition, the pretreatment with nerve growth factor or lithium chloride, a glycogen synthase kinase-3 inhibitor, suppressed the cyclin D1 down-regulation caused by MPP+. Our results demonstrate that MPP+ induces cell cycle arrest independently of its mitochondrial toxicity or the p53 status of the target cells, but rather through the proteasome- and phosphatidylinositol 3-Akt-glycogen synthase kinase-3-dependent cyclin D1 degradation. | |||||
書誌情報 |
The Journal of Biological Chemistry 巻 279, 号 37, p. 38710-38714, 発行日 2004-09 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0021-9258 |