{"created":"2023-05-15T14:34:11.342751+00:00","id":44246,"links":{},"metadata":{"_buckets":{"deposit":"f16dcd46-5c43-40ef-b5e5-6d17afdfdff3"},"_deposit":{"created_by":1,"id":"44246","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"44246"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00044246","sets":["1"]},"author_link":["439613","439611","439612","439610","439614","439609","439607","439608"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"37","bibliographicPageEnd":"38714","bibliographicPageStart":"38710","bibliographicVolumeNumber":"279","bibliographic_titles":[{"bibliographic_title":"The Journal of Biological Chemistry"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"1-Methyl-4-phenylpyridinium ion (MPP+), an active metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, induces cell death and inhibition of cell proliferation in various cells. However, the mechanism whereby MPP+ inhibits cell proliferation is still unclear. In this study, we found that MPP+ suppressed the proliferation with accumulation in G1 phase without inducing cell death in p53-deficient MG63 osteosarcoma cells. MPP+ induced hypophosphorylation of retinoblastoma protein and rapidly down-regulated the protein but not mRNA levels of cyclin D1 in MG63 cells. The down-regulation of cyclin D1 protein was suppressed by a proteasome inhibitor, MG132. The cyclin D1 down-regulation by MPP+ was also observed in p53-positive PC12, HeLa S3, and HeLa 0 cells, which are a subclone of HeLa S3 lacking mitochondrial DNA. Moreover, MPP+ dephosphorylated Akt in PC12 cells, which was rescued by the pretreatment with nerve growth factor. In addition, the pretreatment with nerve growth factor or lithium chloride, a glycogen synthase kinase-3 inhibitor, suppressed the cyclin D1 down-regulation caused by MPP+. Our results demonstrate that MPP+ induces cell cycle arrest independently of its mitochondrial toxicity or the p53 status of the target cells, but rather through the proteasome- and phosphatidylinositol 3-Akt-glycogen synthase kinase-3-dependent cyclin D1 degradation.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9258","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Jie, Bai"}],"nameIdentifiers":[{"nameIdentifier":"439607","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakamura, Hajime"}],"nameIdentifiers":[{"nameIdentifier":"439608","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ueda, Shugo"}],"nameIdentifiers":[{"nameIdentifier":"439609","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kwon, Yong-Won"}],"nameIdentifiers":[{"nameIdentifier":"439610","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Toru"}],"nameIdentifiers":[{"nameIdentifier":"439611","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ban, Sadayuki"}],"nameIdentifiers":[{"nameIdentifier":"439612","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yodoi, Junji"}],"nameIdentifiers":[{"nameIdentifier":"439613","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伴 貞幸","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"439614","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Proteasome-dependent Degradation of Cyclin D1 in 1-Methyl-4-phenylpyridinium Ion (MPP+)-induced Cell Cycle Arrest","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Proteasome-dependent Degradation of Cyclin D1 in 1-Methyl-4-phenylpyridinium Ion (MPP+)-induced Cell Cycle Arrest"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-06-13"},"publish_date":"2006-06-13","publish_status":"0","recid":"44246","relation_version_is_last":true,"title":["Proteasome-dependent Degradation of Cyclin D1 in 1-Methyl-4-phenylpyridinium Ion (MPP+)-induced Cell Cycle Arrest"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:17:43.478154+00:00"}