{"created":"2023-05-15T15:03:33.341200+00:00","id":86106,"links":{},"metadata":{"_buckets":{"deposit":"a36ab297-4e49-414e-a893-5fb55eceaa9e"},"_deposit":{"created_by":1,"id":"86106","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"86106"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00086106","sets":["10:28"]},"author_link":["1045702","1045703"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2022-03-18","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In the avian spinal cord, ten pairs of protrusions called accessory lobes (ALs) are located at both lateral sides of the lumbosacral spinal cords near the dentate ligament. It is predicted that ALs act as a sensory organ of equilibrium during bipedal walking. Previously, we demonstrated that cells isolated from chick ALs showed voltage-gated Na+ and K+ currents and action potentials using electrophysiological method. However, it is unclear which isoform of voltage-gated sodium channel (VGSC) are expressed in ALs and whether cells having neuronal morphology in ALs express VGSCs. To clear up these points, we performed RT-PCR and immunohistochemical experiments. In ALs, the specific PCR products of Nav 1.1–1.7 were detected by RT-PCR analysis, and Nav 1.1 and 1.6 isoforms showed strong intensities among them. In immunohistochemical experiments, cytoplasm and/or cell membranes of the AL contained cells having neuron-like morphology were stained by anti-VGSC antibody and filament-like structure showed GFAP-like immunoreactivity. The VGSC- and GFAP-like immunoreactivities did not overlap. These results show that neuronal-type VGSCs are mainly expressed and that neuron-like morphology cells express VGSCs in the ALs. Our present and previous findings indicate that AL neurons generate action potentials and support the hypothesis that ALs act as organ sensing body balance during walking on the ground.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第99回日本生理学会大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yumi, Matsushita"}],"nameIdentifiers":[{"nameIdentifier":"1045702","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yumi, Matsushita","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1045703","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Neuron-like cells expressing the neuronal-type voltage gated sodium channels are present in the chick spinal accessory lobes.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Neuron-like cells expressing the neuronal-type voltage gated sodium channels are present in the chick spinal accessory lobes."},{"subitem_title":"ニワトリ脊髄accessory lobesには神経型電位依存性ナトリウムチャネルを発現する神経様細胞が存在する","subitem_title_language":"en"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-11"},"publish_date":"2022-05-11","publish_status":"0","recid":"86106","relation_version_is_last":true,"title":["Neuron-like cells expressing the neuronal-type voltage gated sodium channels are present in the chick spinal accessory lobes."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T17:15:28.568851+00:00"}