@article{oai:repo.qst.go.jp:00085291,
 author = {Yutaka, Ono and Satoshi, Kitamura and Katsuya, Sato and Yutaka, Ono and Satoshi, Kitamura and Katsuya, Sato},
 journal = {QST Takasaki Annual Report 2020},
 month = {Mar},
 note = {To establish an easy and quick method for evaluating frequency of mutations occurred in plant DNA, we have tried to apply maximum-depth sequencing (MDS), an extremely deep sequencing method that enables to detect extremely rare mutations through next generation sequencing (NGS) with an error correction. The procedure of the MDS was previously described. Briefly, each molecule of a target DNA extracted from plants was labelled with a distinct DNA barcode, lineally amplified using a single primer and DNA polymerase, and subjected to NGS. Because original mutations that existed in plant DNA will be conserved among NGS reads harboring identical barcodes, they can be distinguished from erroneous mutations caused by PCR or NGS. In this work, we have prepared 4 test reactions to test 1) dual targeting in one reaction, 2) effect of pre-treatment of DNA repair enzymes, and 3) sensitivity of this method by inputting low amount of DNA with a known mutation.},
 title = {Consideration of conditions for extremely deep  sequencing to detect mutations in plant DNA},
 year = {2022}
}