@article{oai:repo.qst.go.jp:00085283,
 author = {Asamizu, Shumpei and Lei, Yukun and Ishizuka, Takumi and Yanagisawa, Masaomi and Katsuya, Sato and Yutaka, Ono and Katsuya, Sato and Yutaka, Ono},
 journal = {QST Takasaki Annual Report 2020},
 month = {Mar},
 note = {We have studied about the bacterial interaction induced production of secondary metabolites by actinomycetes. Tsukamurella pulmonis TP-B0596 (Tp) had been shown to possess ability to induce production of secondary metabolites by Streptomyces species, which are not detected or poorly produced in a mono-culture. Until now, more than 30 new compounds had been isolated from the co-culture with various actinomycetes and Tp. Object of this study is to elucidate the gene(s) which are involved in the regulation of secondary metabolism caused by bacterial interaction. Elucidation of the mechanism can lead to the fundamental understanding of bacterial interaction induced secondary metabolism, as well as application for genetic tools to discover novel bioactive natural products from untapped genes. We had employed carbon ion beams induced mutation to investigated the gene(s) responsible for the responsive production of secondary metabolism induced by Tp. Using Streptomyces coelicolor JCM4020, random spore mutant library was generated. Undecylprodigiosin (RED) production deficient mutants was screened by mixing with Tp on agar plates. Through this screening, 59 mutants from around 152,000 tested spores were obtained. The genome re-sequence and gene complementation studies were revealed that molybdopterin biosynthetic enzyme, and TetR-like transcriptional regulator are also responsible for the phenotype. We further analyzed the involvement of TetR-like transcriptional regulator by targeted gene disruption in S. coelicolor A3(2) and confirmed the delayed production of RED.},
 title = {Characterization of ABC transporter which cause the delayed contact-dependent RED production in Streptomyces coelicolor},
 volume = {QST-M-33},
 year = {2022}
}