@article{oai:repo.qst.go.jp:00085282,
 author = {Den, Hiroki and Tatsumoto, Kouji and Futenma, Akira and Maeda, Nobuhiko and Asada, Ryoko and Sakamoto, Jin and Tsuchido, Tetsuaki and Furuta, Masakazu and Katsuya, Sato and Yutaka, Ono and Katsuya, Sato and Yutaka, Ono},
 journal = {QST Takasaki Annual Report 2020},
 month = {Mar},
 note = {Bacterial spores of the genus Bacillus are more resistant to physical and chemical stresses than their vegetative cells due to their unique layered and dehydrated interior structure. In order to use radiation sterilization effectively for food and medical products, it is essential to clarify the effect of radiation on resistant spores and to clarify the characteristics of radiation irradiation in comparison with other sterilization methods, in order to improve the sterilization method and to combine it with other sterilization methods. In this study, we investigated the role of DNA repair in the resistance of spores to gamma-irradiation and ion beam irradiation of different LETs, and the mechanism and developmental dynamics of damaged spores. The killing effect of ion beam irradiation of spores with different LETs showed a LET-dependent loss of colony forming ability. Analysis by DiVSaL method, an evaluation method of injured cell population based on comparison of time before starting vegetative growth of irradiated cells with unirradiated cells, indicated the increase of injured cell population with increasing dose. The ratio of the injured cell population tends to increase with increasing RBE according to ion species and energies used for irradiation. These results suggest that capacity of repairing the DNA damages within the spores would prevent injured cell formation during the recovering process from the both ion beams and gamma irradiation.},
 title = {Evaluation of damage and growth inhibition of Bacillus subtilis spores by analysis of their growth curves after irradiation of gamma-rays and high-LET ion beams},
 volume = {QST-M-33},
 year = {2022}
}