@article{oai:repo.qst.go.jp:00085020,
 author = {Saito, Yutaro and Yatabe, Hiroyuki and Tamura, Iori and Kondo, Yohei and Ishida, Ryo and Seki, Tomohiro and Hiraga, Keita and Eguchi, Akihiro and Takakusagi, Yoichi and Saito, Keisuke and Oshima, Nobu and Ishikita, Hiroshi and Yamamoto, Kazutoshi and C. Krishna, Murali and Sando, Shinsuke and Yoichi, Takakusagi},
 issue = {13},
 journal = {Sci Adv},
 month = {Mar},
 note = {Dynamic nuclear polarization (DNP) is a cutting-edge technique that dramatically enhances the detection sensitivity of molecules using nuclear magnetic resonance imaging (NMR/MRI). This methodology enables real-time imaging of dynamic metabolic status in vivo using MRI. To expand the targetable metabolic reactions, there is a demand for developing exogenous, i.e., artificially designed, DNP-NMR molecular probes; however, complying with the requirements of practical DNP-NMR molecular probes is challenging due to the lack of established design guidelines. Herein, we report Ala-[1-13C]Gly-d2-NMe2 as a DNP-NMR molecular probe for in vivo detection of aminopeptidase N (APN) activity. We developed this probe rationally through precise structural investigation, calculation, biochemical assessment, and advanced molecular design to achieve rapid and detectable responses to enzyme activity in vivo. With the fabricated probe, we successfully detected enzymatic activity in vivo. This report presents a comprehensive approach for the development of artificially derived, practical DNP-NMR molecular probes through structure-guided molecular design.},
 title = {Structure-guided design enables development of a hyperpolarized molecular probe for detection of aminopeptidase N activity  in vivo},
 volume = {8},
 year = {2022}
}