{"created":"2023-05-15T15:02:42.212304+00:00","id":85014,"links":{},"metadata":{"_buckets":{"deposit":"99334606-76b8-43d6-a014-05aafecdff04"},"_deposit":{"created_by":1,"id":"85014","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"85014"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00085014","sets":["1"]},"author_link":["1040762","1040761","1040763"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"42","bibliographicPageStart":"e2114412118","bibliographicVolumeNumber":"118","bibliographic_titles":[{"bibliographic_title":"PNAS"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The low-complexity (LC) domain of the fused in sarcoma (FUS) RNA binding protein self-associates in a manner causing phase separation from an aqueous environment. Incubation of the FUS LC domain under physiologically normal conditions of salt and pH leads to rapid formation of liquid-like droplets that mature into a gel-like state. Both examples of phase separation have enabled reductionist biochemical assays allowing discovery of an N-terminal region of 57 residues that assembles into a labile, cross-β structure. Here we provide evidence of a nonoverlapping, C-terminal region of the FUS LC domain that also forms specific cross-β interactions. We propose that biologic function of the FUS LC domain may operate via the mutually exclusive use of these N- and C-terminal cross-β cores. Neurodegenerative disease–causing mutations in the FUS LC domain are shown to imbalance the two cross-β cores, offering an unanticipated concept of LC domain function and dysfunction.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1073/pnas.2114412118","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.pnas.org/content/118/42/e2114412118","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0027-8424","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Masato, Kato"}],"nameIdentifiers":[{"nameIdentifier":"1040761","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"L. McKnight, Steven"}],"nameIdentifiers":[{"nameIdentifier":"1040762","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Masato, Kato","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1040763","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The low-complexity domain of the FUS RNA binding protein self-assembles via the mutually exclusive use of two distinct cross-β cores","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The low-complexity domain of the FUS RNA binding protein self-assembles via the mutually exclusive use of two distinct cross-β cores"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-02-16"},"publish_date":"2022-02-16","publish_status":"0","recid":"85014","relation_version_is_last":true,"title":["The low-complexity domain of the FUS RNA binding protein self-assembles via the mutually exclusive use of two distinct cross-β cores"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T17:24:34.428432+00:00"}