@article{oai:repo.qst.go.jp:00084970,
 author = {Okazaki, Yasumasa and Sasaki, Kanako and Ito, Nanami and Tanaka, Hiromasa and Ken-ichiro, Matsumoto and Hori, Masaru and Toyokuni, Shinya and Kenichiro, Matsumoto},
 issue = {1},
 journal = {Free Radical Research},
 month = {Jan},
 note = {Non-thermal plasma (NTP) devices have been explored for medical applications. NTP devices discharge electrons, positive ions, ultraviolet and reactive oxygen species (ROS) and reactive nitrogen species (RNS), such as hydroxyl radical (●OH), singlet oxygen (1O2), superoxide (O2●-), hydrogen peroxide (H2O2), ozone and nitric oxide at near physiological temperature. In preclinical stages or human clinical trials, NTP promotes blood coagulation, eradication of bacterial, viral and biofilm-related infections, wound healing and cancer cell death. Here, we detected ferric, vanadium and gold(III) ions significantly elevated lipid peroxidation, which were measured by 2-thiobarbituric acid-reactive substances (TBARS) in combination with NTP exposure. Using 3,3,5,5-tetramethyl-1-pyrroline-N-oxide (M4PO) as a spin probe, we identified tetrachloroaurate (III) yielded M4PO-X spin adduct with electron paramagnetic resonance (EPR). Tetrachloroaurate-induced oxidation was attenuated efficiently by reduced glutathione (GSH) and oxidized (GSSG), while glycine (Gly) and L-glutamate (Glu), which are constituent parts of GSH, were ineffective. Furthermore, GSH and GSSG efficiently suppressed tetrachloroaurate-induced lipid peroxidation, while Gly and Glu were also ineffective to suppress the elevation of TBARS. These results indicate that tetrachloroaurate-induced oxidation were attenuated by GSH as well as GSSG. Further studies were warranted to elucidate the redox reactions between metal ions and biomolecules that put forward the clinical application of NTP.},
 title = {Tetrachloroaurate (III)–induced oxidation increases non-thermal plasma-induced oxidative stress},
 volume = {56},
 year = {2022}
}