@article{oai:repo.qst.go.jp:00084836,
 author = {Du, Junyan and Shun-Ichiro, Kageyama and Hirata, Hidenari and Motegi, Atsushi and Nakamura, Masaki and Hirano, Yasuhiro and Okumura, Masayuki and Yamashita, Riu and Tsuchihara, Katsuya and Hojo, Hidehiro and Hirayama, Ryoichi and Akimoto, Tetsuo and Shunichiro, Kageyama and Ryoichi, Hirayama},
 journal = {Biochemical and biophysical research communications},
 month = {Jan},
 note = {Radiotherapy (RT) is an effective treatment option for cancer; however, its efficacy remains less than optimal in locally advanced cancer. Immune checkpoint inhibitor-based therapy, including the administration of anti-PD-L1 antibodies, is a promising approach that works synergistically with RT. Proton beam therapy and carbon-ion therapy are common options for patients with cancer. Proton and carbon ions are reported to induce an immune reaction in cancer cells; however, the underlying mechanisms remain unclear. Here, we aimed to compare the immune responses after irradiation (IR) with X-ray, protons, and carbon ions in an oesophageal cancer cell line and the underlying mechanisms. An oesophageal cancer cell line, KYSE450, was irradiated with 1 fraction/15 GyE (Gy equivalent) of X-ray, proton, or carbon-ion beams, and then, the cells were harvested for RNA sequencing and gene enrichment analysis. We also knocked out STING and STAT1 in the quest for mechanistic insights. RNA sequencing data revealed that gene expression signatures and biological processes were different in KYSE450 irradiated with X-ray, proton, and carbon-ion beams 6-24 h after IR. However, after 3 days, a common gene expression signature was detected, associated with biological pathways involved in innate immune responses. Gene knock-out experiments revealed that the STING-STAT1 axis underlies the immune reactions after IR. X-Ray, proton, and carbon-ion IRs induced similar immune responses, regulated by the STING-STAT1 axis.},
 pages = {55--60},
 title = {Comparative analysis of the immune responses in cancer cells irradiated with X-ray, proton and carbon-ion beams.},
 volume = {585},
 year = {2021}
}