@article{oai:repo.qst.go.jp:00084592,
 author = {Tsutsui, Makusu and Yokota, Kazumichi and Arima, Akihide and Washio, Takashi and Yoshinobu, Baba and Kawai, Tomoji and Yoshinobu, Baba},
 issue = {9},
 journal = {Small Methods},
 month = {Sep},
 note = {Amplification-free genome analysis can revolutionize biology and medicine by uncovering genetic variations among individuals. Here, the authors report on a 3D-integrated nanopore for electrolysis to in situ detection of single-molecule DNA in a cell by ionic current measurements. It consists of a SiO2 multipore sheet and a SiNx nanopore membrane stacked vertically on a Si wafer. Single cell lysis is demonstrated by 106 V m−1-level electrostatic field focused at the multinanopore. The intracellular molecules are then directly detected as they move through a sensing zone, wherein the authors find telegraphic current signatures reflecting folding degrees of freedom of the millimeter-long polynucleotides threaded through the SiNx nanopore. The present device concept may enable on-chip single-molecule sequencing to multi-omics analyses at a single-cell level.},
 title = {Detecting Single Molecule Deoxyribonucleic Acid in a Cell Using a Three-Dimensionally Integrated Nanopore},
 volume = {5},
 year = {2021}
}