{"created":"2023-05-15T15:02:22.436359+00:00","id":84575,"links":{},"metadata":{"_buckets":{"deposit":"5da08548-a5c3-4fa4-ab5b-2b8a7c9fc7a0"},"_deposit":{"created_by":1,"id":"84575","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"84575"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00084575","sets":["1"]},"author_link":["1020736","1020735","1020745","1020738","1020737","1020740","1020741","1020743","1020746","1020747","1020739","1020742","1020744"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"15","bibliographicPageStart":"3833","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"Cancers "}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hypoxia-Inducible Factor 1α (HIF-1α), which promotes cancer cell survival, is the main regulator of oxygen homeostasis. Hypoxia combined with photon and carbon ion irradiation (C-ions) stabilizes HIF-1α. Silencing HIF-1α under hypoxia leads to substantial radiosensitization of Head-and-Neck Squamous Cell Carcinoma (HNSCC) cells after both photons and C-ions. Thus, this study aimed to clarify a potential involvement of HIF-1α in the detection, signaling, and repair of DNA Double-Strand-Breaks (DSBs) in response to both irradiations, in two HNSCC cell lines and their subpopulations of Cancer-Stem Cells (CSCs). After confirming the nucleoshuttling of HIF-1α in response to both exposure under hypoxia, we showed that silencing HIF-1α in non-CSCs and CSCs decreased the initiation of the DSB detection (P-ATM), and increased the residual phosphorylated H2AX (γH2AX) foci. While HIF-1α silencing did not modulate 53BP1 expression, P-DNA-PKcs (NHEJ-c) and RAD51 (HR) signals decreased. Altogether, our experiments demonstrate the involvement of HIF-1α in the detection and signaling of DSBs, but also in the main repair pathways (NHEJ-c and HR), without favoring one of them. Combining HIF-1α silencing with both types of radiation could therefore present a potential therapeutic benefit of targeting CSCs mostly present in tumor hypoxic niches.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/cancers13153833","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.mdpi.com/2072-6694/13/15/3833","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2072-6694","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Anne-Sophie, Wozny"}],"nameIdentifiers":[{"nameIdentifier":"1020735","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Gauthier , Arnaud"}],"nameIdentifiers":[{"nameIdentifier":"1020736","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Alphonse, Gersende"}],"nameIdentifiers":[{"nameIdentifier":"1020737","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Céline, Malésys"}],"nameIdentifiers":[{"nameIdentifier":"1020738","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Varoclier, Virginie"}],"nameIdentifiers":[{"nameIdentifier":"1020739","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Beuve, Michael"}],"nameIdentifiers":[{"nameIdentifier":"1020740","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Delphine, Brichart-Vernos"}],"nameIdentifiers":[{"nameIdentifier":"1020741","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nicolas, Magné"}],"nameIdentifiers":[{"nameIdentifier":"1020742","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Vial, Nicolas"}],"nameIdentifiers":[{"nameIdentifier":"1020743","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ardail, Dominique"}],"nameIdentifiers":[{"nameIdentifier":"1020744","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tetsuo, Nakajima"}],"nameIdentifiers":[{"nameIdentifier":"1020745","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Claire, Rodriguez-Lafrasse"}],"nameIdentifiers":[{"nameIdentifier":"1020746","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tetsuo, Nakajima","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1020747","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Involvement of HIF-1α in the Detection, Signaling, and Repair of DNA Double-Strand Breaks after Photon and Carbon-Ion Irradiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Involvement of HIF-1α in the Detection, Signaling, and Repair of DNA Double-Strand Breaks after Photon and Carbon-Ion Irradiation"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-10-05"},"publish_date":"2021-10-05","publish_status":"0","recid":"84575","relation_version_is_last":true,"title":["Involvement of HIF-1α in the Detection, Signaling, and Repair of DNA Double-Strand Breaks after Photon and Carbon-Ion Irradiation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:20:14.903018+00:00"}