{"created":"2023-05-15T15:01:58.257784+00:00","id":84054,"links":{},"metadata":{"_buckets":{"deposit":"9c48da5b-2810-4004-ab18-4df6f8eec6ad"},"_deposit":{"created_by":1,"id":"84054","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"84054"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00084054","sets":["1"]},"author_link":["1015142","1015140","1015145","1015138","1015143","1015141","1015137","1015139","1015144"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageStart":"848","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"Plants"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Argon-ion beam is an effective mutagen capable of inducing a variety of mutation types. In this study, an argon ion-induced pale green mutant of  was isolated and characterized. The mutant, designated Ar50-33-pg1, exhibited moderate defects of growth and greening and exhibited rapid chlorosis in photosynthetic tissues. Fluorescence microscopy confirmed that mesophyll chloroplasts underwent substantial shrinkage during the chlorotic process. Genetic and whole-genome resequencing analyses revealed that Ar50-33-pg1 contained a large 940 kb deletion in chromosome V that encompassed more than 100 annotated genes, including 41 protein-coding genes such as /, , and . One of the deleted genes, , for a thylakoid membrane-localized metalloprotease, was the major contributory gene responsible for the pale mutant phenotype. Both an  mutant and F progeny of an Ar50-33-pg1 ×  cross-exhibited chlorotic phenotypes similar to those of Ar50-33-pg1. Furthermore, ultrastructural analysis of mesophyll cells revealed that Ar50-33-pg1 and  initially developed wild type-like chloroplasts, but these were rapidly disassembled, resulting in thylakoid disorganization and fragmentation, as well as plastoglobule accumulation, as terminal phenotypes. Together, these data support the utility of heavy-ion mutagenesis for plant genetic analysis and highlight the importance of  in the structural maintenance of grana in mesophyll chloroplasts.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"33922223","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/plants10050848","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.mdpi.com/2223-7747/10/5/848","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2223-7747","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sanjaya, Alvin"}],"nameIdentifiers":[{"nameIdentifier":"1015137","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kazama, Yusuke"}],"nameIdentifiers":[{"nameIdentifier":"1015138","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishii, Kotaro"}],"nameIdentifiers":[{"nameIdentifier":"1015139","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Muramatsu, Ryohsuke"}],"nameIdentifiers":[{"nameIdentifier":"1015140","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kanamaru, Kengo"}],"nameIdentifiers":[{"nameIdentifier":"1015141","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohbu, Sumie"}],"nameIdentifiers":[{"nameIdentifier":"1015142","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Abe, Tomoko"}],"nameIdentifiers":[{"nameIdentifier":"1015143","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"T Fujiwara, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"1015144","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kotaro, Ishii","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1015145","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An Argon-Ion-Induced Pale Green Mutant of Arabidopsis Exhibiting Rapid Disassembly of Mesophyll Chloroplast Grana","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An Argon-Ion-Induced Pale Green Mutant of Arabidopsis Exhibiting Rapid Disassembly of Mesophyll Chloroplast Grana"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-07-14"},"publish_date":"2021-07-14","publish_status":"0","recid":"84054","relation_version_is_last":true,"title":["An Argon-Ion-Induced Pale Green Mutant of Arabidopsis Exhibiting Rapid Disassembly of Mesophyll Chloroplast Grana"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:44:29.374051+00:00"}