@article{oai:repo.qst.go.jp:00084054,
 author = {Sanjaya, Alvin and Kazama, Yusuke and Ishii, Kotaro and Muramatsu, Ryohsuke and Kanamaru, Kengo and Ohbu, Sumie and Abe, Tomoko and T Fujiwara, Makoto and Kotaro, Ishii},
 issue = {5},
 journal = {Plants},
 month = {Jan},
 note = {Argon-ion beam is an effective mutagen capable of inducing a variety of mutation types. In this study, an argon ion-induced pale green mutant of  was isolated and characterized. The mutant, designated Ar50-33-pg1, exhibited moderate defects of growth and greening and exhibited rapid chlorosis in photosynthetic tissues. Fluorescence microscopy confirmed that mesophyll chloroplasts underwent substantial shrinkage during the chlorotic process. Genetic and whole-genome resequencing analyses revealed that Ar50-33-pg1 contained a large 940 kb deletion in chromosome V that encompassed more than 100 annotated genes, including 41 protein-coding genes such as /, , and . One of the deleted genes, , for a thylakoid membrane-localized metalloprotease, was the major contributory gene responsible for the pale mutant phenotype. Both an  mutant and F progeny of an Ar50-33-pg1 ×  cross-exhibited chlorotic phenotypes similar to those of Ar50-33-pg1. Furthermore, ultrastructural analysis of mesophyll cells revealed that Ar50-33-pg1 and  initially developed wild type-like chloroplasts, but these were rapidly disassembled, resulting in thylakoid disorganization and fragmentation, as well as plastoglobule accumulation, as terminal phenotypes. Together, these data support the utility of heavy-ion mutagenesis for plant genetic analysis and highlight the importance of  in the structural maintenance of grana in mesophyll chloroplasts.},
 title = {An Argon-Ion-Induced Pale Green Mutant of Arabidopsis Exhibiting Rapid Disassembly of Mesophyll Chloroplast Grana},
 volume = {10},
 year = {2021}
}