@misc{oai:repo.qst.go.jp:00083972, author = {Tomoko, Sunami and Hidetoshi, Kono and Tomoko, Sunami and Hidetoshi, Kono}, month = {Nov}, note = {The first nucleosomes positioned around the transcription start site, +1 nucleosomes, play critical roles in transcriptional regulation. We previously exhibited that AA/TT is enriched in the entry sites of +1 nucleosomes in yeast, and AA/TT regions in nucleosomes are more susceptible to MNase. To further understand the molecular mechanisms, we performed FRET analysis of nucleosomes reconstituted with and without A-tract at the entry/exit sites. Regardless of the sequences, nucleosomes show similar amount of the wrapped state in low salt concentrations. However, we found salt-induced DNA unwrapping occurs more easily in the A-tract nucleosomes, indicating the transition energy from the wrapped to unwrapped state might be lower for the A-tract nucleosomes., 第59回 日本生物物理学会年会}, title = {Sequence dependence of nucleosomal DNA unwrapping}, year = {2021} }