{"created":"2023-05-15T15:01:48.680142+00:00","id":83841,"links":{},"metadata":{"_buckets":{"deposit":"c1aee68e-61cf-41ef-94df-09a26b40b3f6"},"_deposit":{"created_by":1,"id":"83841","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"83841"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00083841","sets":["1"]},"author_link":["1011495","1011493","1011492","1011491","1011494","1011496","1011497"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageStart":"119021","bibliographicVolumeNumber":"246","bibliographic_titles":[{"bibliographic_title":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In this study, a facile, sensitive, and precise lab-on-a-chip electrophoretic method coupled with light-emitting diode induced fluorescence (LED-IF) detection was developed to assay three antiepileptic drugs, namely, vigabatrin, pregabalin, and gabapentin, in pharmaceutical formulations. The analytes were derivatised offline for the first time with fluorescine-5-isothiocyanate (FITC) to yield highly fluorescent derivatives with λex/em of 490/520 nm. The FITC-labelled analytes were injected, separated, and quantitated by a microfluidic electrophoresis device using fluorescence detection. The labelled analytes were monitored using a blue LED-IF system. The separation conditions were significantly optimised adding specific concentrations of heptakis-(2,6-di-O-methyl)-β-cyclodextrin (HDM-β-CD) and methylcellulose to the buffer solution (40 mM borate buffer). HDM-β-CD acted as a selective host for the studied antiepileptic drugs, rendering a high separation efficiency. Methylcellulose was used as an efficient dynamic coating polymer to prevent the labelled drugs from being adsorbed on the inner surfaces of the poly (methylmethacrylate) microchannels. A laboratory-prepared ternary mixture of the three antiepileptic drugs was separated within 100 s with acceptable resolution between all the three analytes (Rs > 3) and a high number of theoretical plates (N) for each analyte (N ≈ 106 plates/m). The sensitivity of the method was enhanced approximately 80-fold by stacking to yield a detection limit below 0.6 ng mL−1 in the concentration range of 2.0–200.0 ng mL−1. The method was successfully validated for analysing the studied drugs in their pharmaceutical formulations.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.saa.2020.119021","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/abs/pii/S1386142520310003?via%3Dihub#!","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1386-1425","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"M.Zeid, Abdallah"}],"nameIdentifiers":[{"nameIdentifier":"1011491","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Jeehan M. Nasr, Jenny"}],"nameIdentifiers":[{"nameIdentifier":"1011492","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Belal, Fathalla"}],"nameIdentifiers":[{"nameIdentifier":"1011493","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"I. Walash, Mohamed"}],"nameIdentifiers":[{"nameIdentifier":"1011494","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshinobu, Baba"}],"nameIdentifiers":[{"nameIdentifier":"1011495","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kaji, Noritada"}],"nameIdentifiers":[{"nameIdentifier":"1011496","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshinobu, Baba","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1011497","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Determination of three antiepileptic drugs in pharmaceutical formulations using microfluidic chips coupled with light-emitting diode induced fluorescence detection","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Determination of three antiepileptic drugs in pharmaceutical formulations using microfluidic chips coupled with light-emitting diode induced fluorescence detection"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-09-29"},"publish_date":"2021-09-29","publish_status":"0","recid":"83841","relation_version_is_last":true,"title":["Determination of three antiepileptic drugs in pharmaceutical formulations using microfluidic chips coupled with light-emitting diode induced fluorescence detection"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:59:51.025242+00:00"}