{"created":"2023-05-15T15:01:46.086249+00:00","id":83783,"links":{},"metadata":{"_buckets":{"deposit":"20ab3fce-4cd4-462f-9bb3-36118013b930"},"_deposit":{"created_by":1,"id":"83783","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"83783"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00083783","sets":["10:29"]},"author_link":["1010371","1010372"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2021-12-07","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The cell membrane is a nanoscale 2D fluid crystalline assembly with sub-compartment domains that are critical for cellular functions, including transport of molecules, communications, and metabolic properties with its external medium [1]. These domains are distinguished by different phases of lipid membranes, and the fluidity of the lipid bilayer, described by the 2D translational diffusion of lipid molecules [2], determines the most fundamental property of lipids in different phases and therefore domains. Nanoscale NMR and correlation spectroscopy using a NV center has emerged as a quantum measurement platform that allows for label-free diffusion measurement with nuclear spin from a small detection volume of ~ (6 nm) 3 [3].\nIn this study, we investigate nanoscale phase change detection of lipid bilayers utilizing ensemble-averaged nuclear spin detection from small volume ~ (6 nm)3, which is determined by the depth of the NV center. Analysis of nanoscale NMR signal confirms thickness of lipid bilayer to be 6.2 nm ± 3.4 nm with proton density of 65 proton/nm3 on top of diamond sample. The result of the correlation spectroscopy was compared with the 2D molecular diffusion model constructed by Monte Carlo simulation combined with results from molecular dynamics (MD) simulation. There is a change in diffusion constant from 1.5 ± 0.25 nm2/μsec to 3.0 ± 0.5 nm2/μsec when the temperature changes from 26.5 °C to 36.0 °C [4]. Our results demonstrate that label-free detection of changes in translational diffusion of lipid molecules is possible using nanoscale diamond magnetometry. Direct observation of changes in the diffusion constant paves the way for the label-free identification of domains that are formed in the cell membrane to understand the relationship between cell membrane dynamics and cell function [5].\n[1] G. van Meer, A. I. P. M. de Kroon, J Cell Sci. 2011, 124, 5-8.\n[2] G. van Meer, D. R. Voelker, G. W. Feigenson, Nat. Rev. Mol. Cell Biol. 2008, 9, 112-124.\n[3] T. Staudacher, N. Raatz, S. Pezzagna, J. Meijer, F. Reinhard, C. A. Meriles, J. Wrachtrup, Nat. Comm. 2015, 6, 8527.\n[4] H. Ishiwata, H. C. Watanabe, S. Hanashima, T. Iwasaki, M. Hatano, Advanced Quantum Technology 2021, 4, 2000106.\n[5] M. A. Pavel, E. N. Petersen, H. Wang, R. A. Lerner, S. B. Hansen, Proc. Natl. Acad. Sci. USA. 2020, 117, 13757-13766.\n","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"2021 MRS Fall Meeting & Exhibit","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hitoshi, Ishiwata"}],"nameIdentifiers":[{"nameIdentifier":"1010371","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hitoshi, Ishiwata","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1010372","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Label-Free Phase Change Detection of Lipid Bilayers Using Nanoscale Diamond Magnetometry","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Label-Free Phase Change Detection of Lipid Bilayers Using Nanoscale Diamond Magnetometry"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-11-10"},"publish_date":"2021-11-10","publish_status":"0","recid":"83783","relation_version_is_last":true,"title":["Label-Free Phase Change Detection of Lipid Bilayers Using Nanoscale Diamond Magnetometry"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:04:13.972855+00:00"}