{"created":"2023-05-15T15:01:41.533546+00:00","id":83682,"links":{},"metadata":{"_buckets":{"deposit":"4eab1717-634f-4f88-a783-de4b171f89e9"},"_deposit":{"created_by":1,"id":"83682","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"83682"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00083682","sets":["10:29"]},"author_link":["1008926","1008917","1008918","1008920","1008925","1008919","1008923","1008924","1008921","1008922"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2021-10-20","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aim/Introduction: Auger electrons (Auger e-\n) have the \npotential for therapeutic applications by inducing nanoscale physiochemical damage to biomolecules due to \ntheir short-range (2-500 nm). Although DNA is the primary \ntarget of Auger e-\n, it remains challenging to maximize the \ninteraction between Auger e-\n and DNA. To assess the DNAdamaging efect of Auger e-\n released as close as possible \nto DNA, we focused on a platinum(Pt)-based antineoplastic \ndrug, cisplatin, because it can form direct DNA adducts \nbetween Pt and nucleobases. Here, to evaluate the efect of \nAuger e-\n without chemical factors we radio-synthesized nocarrier-added (n.c.a.) [189, 191Pt]cisplatin (radio-cisplatin), and \ninvestigated its in vitro properties and DNA-damaging efect \nin cultured cells. Materials and Methods: N.c.a. radio-cisplatin \nwas radio-synthesized by treating n.c.a. radio-PtCl4\n2- with \nexcess NH3\n and heating the reaction mixture, and the product \nwas isolated by preparative HPLC. As in vitro evaluation of \nradio-cisplatin, cellular uptake, intracellular distribution, and \nDNA binding were investigated, and DNA double-strand \nbreaks (DSBs) were evaluated by immunofuorescence \nstaining of γH2AX. Results: Our production method provided \nn.c.a. radio-cisplatin with a radiochemical purity of 99% at the \nend of synthesis. Although uptake of radio-cisplatin was low \n(0.6% incubated dose after 25-h incubation), approximately \n20% of intracellular radio-Pt was in a nucleus, and 2% \nof intra-nucleus radio-Pt bound to DNA. Due to the low \naccumulation of radio-Pt, the frequency of cells with γH2AX \nfoci was low (1%) in the γH2AX assays. Nevertheless, strong \nsignals in nuclei of tumor cells treated with radio-cisplatin \nwere found more often than with saline or nonradioactive \ncisplatin, suggesting Auger e-\n released very close to DNA \ncause more DSB. Conclusion: N.c.a. radio-cisplatin was \nsuccessfully prepared at high radiochemical purities as an \nefective tool to evaluate DNA damage induced by Auger \ne-\n without chemical factors. Radio-cisplatin binding to DNA \ncaused severe DSBs by the release of Auger e-\n very close \nto DNA without chemical damage by carriers. References:\nObata, et al. Int. J. Mol. Sci. 2021, 22, 4622. Obata, et al. Sci Rep \n2021, 11, 8140.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"EANM21(34th Annual Congress of the European Association of Nuclear Medicine)","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Honoka, Obata"}],"nameIdentifiers":[{"nameIdentifier":"1008917","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Atsushi, Tsuji"}],"nameIdentifiers":[{"nameIdentifier":"1008918","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kotaro, Nagatsu"}],"nameIdentifiers":[{"nameIdentifier":"1008919","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mikako, Ogawa"}],"nameIdentifiers":[{"nameIdentifier":"1008920","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Ming-Rong"}],"nameIdentifiers":[{"nameIdentifier":"1008921","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Honoka, Obata","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1008922","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Atsushi, Tsuji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1008923","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kotaro, Nagatsu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1008924","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mikako, Ogawa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1008925","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Ming-Rong","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1008926","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Production and in vitro evaluation of no-carrier-added  radio-cisplatin emitting Auger electrons","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Production and in vitro evaluation of no-carrier-added  radio-cisplatin emitting Auger electrons"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-10-07"},"publish_date":"2021-10-07","publish_status":"0","recid":"83682","relation_version_is_last":true,"title":["Production and in vitro evaluation of no-carrier-added  radio-cisplatin emitting Auger electrons"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:10:19.916846+00:00"}