{"created":"2023-05-15T15:01:31.731916+00:00","id":83464,"links":{},"metadata":{"_buckets":{"deposit":"c2489401-5381-4641-9598-834e0ffcb9a0"},"_deposit":{"created_by":1,"id":"83464","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"83464"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00083464","sets":["1"]},"author_link":["1005275","1005287","1005283","1005276","1005286","1005280","1005278","1005277","1005281","1005284","1005279","1005288","1005285","1005282"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"15","bibliographicPageStart":"167110","bibliographicVolumeNumber":"433","bibliographic_titles":[{"bibliographic_title":"Journal of Molecular Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"tetramer of (H3-H4)2, wrapped around by ~145 bp of DNA. Detailed core structures of nucleosomes have been established by X-ray and cryo-EM, however, histone tails have not been visualized. Here, we have examined the dynamic structures of the H2A and H2B tails in 145-bp and 193-bp nucleosomes using NMR, and have compared them with those of the H2A and H2B tail peptides unbound and bound to DNA. Whereas the H2A C-tail adopts a single but different conformation in both nucleosomes, the N-tails of H2A and H2B adopt two distinct conformations in each nucleosome. To clarify these conformations, we conducted molecular dynamics (MD) simulations, which suggest that the H2A N-tail can locate stably in either the major or minor grooves of nucleosomal DNA. While the H2B N-tail, which sticks out between two DNA gyres in the nucleosome, was considered to adopt two different orientations, one toward the entry/exit side and one on the opposite side. Then, the H2A N-tail minor groove conformation was obtained in the H2B opposite side and the H2B N-tail interacts with DNA similarly in both sides, though more varied conformations are obtained in the entry/exit side. Collectively, the NMR findings and MD simulations suggest that the minor groove conformer of the H2A N-tail is likely to contact DNA more strongly than the major groove conformer, and the H2A N-tail reduces contact with DNA in the major groove when the H2B N-tail is located in the entry/exit side.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jmb.2021.167110","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S002228362100334X","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-2836","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ohtomo, Hideaki"}],"nameIdentifiers":[{"nameIdentifier":"1005275","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Jun-ichi, Kurita"}],"nameIdentifiers":[{"nameIdentifier":"1005276","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakuraba, Shun"}],"nameIdentifiers":[{"nameIdentifier":"1005277","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Li, Zhenhai"}],"nameIdentifiers":[{"nameIdentifier":"1005278","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Arimura, Yasuhiro"}],"nameIdentifiers":[{"nameIdentifier":"1005279","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wakamori, Masatoshi"}],"nameIdentifiers":[{"nameIdentifier":"1005280","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsunaka, Yasuo"}],"nameIdentifiers":[{"nameIdentifier":"1005281","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Umehara, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"1005282","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kurumizaka, Hitoshi"}],"nameIdentifiers":[{"nameIdentifier":"1005283","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kono, Hidetoshi"}],"nameIdentifiers":[{"nameIdentifier":"1005284","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishimura, Yoshifumi"}],"nameIdentifiers":[{"nameIdentifier":"1005285","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shun, Sakuraba","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1005286","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Li, Zhenhai","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1005287","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hidetoshi, Kono","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1005288","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The N-terminal Tails of Histones H2A and H2B Adopt Two Distinct Conformations in the Nucleosome with Contact and Reduced Contact to DNA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The N-terminal Tails of Histones H2A and H2B Adopt Two Distinct Conformations in the Nucleosome with Contact and Reduced Contact to DNA"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-07-20"},"publish_date":"2020-07-20","publish_status":"0","recid":"83464","relation_version_is_last":true,"title":["The N-terminal Tails of Histones H2A and H2B Adopt Two Distinct Conformations in the Nucleosome with Contact and Reduced Contact to DNA"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:24:36.736614+00:00"}