@misc{oai:repo.qst.go.jp:00083426,
 author = {長谷, 純宏 and 佐藤, 勝也 and 清藤, 一 and 大野, 豊 and Yoshihiro, Hase and Katsuya, Sato and Hajime, Seito and Yutaka, Ono},
 month = {Sep},
 note = {Chronic gamma irradiation is one of the mutagenesis methods in practical plant mutation breeding, however, little is known about the characteristics of induced mutations at the molecular level. To evaluate the mutagenic effects of chronic gamma irradiation even at low dose rates, mutation accumulation lines were prepared by recurrent irradiation. Seven-day-old Arabidopsis plants were grown under a gamma-ray environment until the plants started bolting (approx. two weeks) at the dose rates of 500 mGy/h, 73–100 mGy/h, 0.6–1.9 mGy/h and 2–4 µGy/h. A significant reduction in seed fertility was only observed at 500 mGy/h. This irradiation process was repeated in five successive generations and the sixth-generation plants were subjected to whole genome resequencing. The mutation frequencies at 500 mGy/h and 73–100 mGy/h were 25 and 4 times higher than that of unirradiated control, respectively. The types of mutations were similar to those observed by the acute gamma irradiation of dry seeds, but the chronic gamma irradiation showed much higher mutation frequencies per dose. No significant increase in mutation frequency was observed for the other two low-dose-rate groups. The transition/transversion ratio decreased as the dose rate increased, and interestingly, the ratio at 0.6–1.9 mGy/h was significantly lower than that in the control. This suggests that the plants may responded to low dose rate of gamma rays around 1 mGy/h, even though the overall mutation frequency did not increase., 日本放射線影響学会第 64 回大会},
 title = {シロイヌナズナにおけるガンマ線緩照射の遺伝的影響},
 year = {2021}
}