{"created":"2023-05-15T15:01:01.874691+00:00","id":82791,"links":{},"metadata":{"_buckets":{"deposit":"f2b78444-ada9-4dd9-9845-f97dd7dce616"},"_deposit":{"created_by":1,"id":"82791","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"82791"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00082791","sets":["1"]},"author_link":["1015923","1015914","1015921","1015909","1015916","1015922","1015912","1015919","1015908","1015913","1015917","1015915","1015920","1015910","1015918","1015911"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageStart":"4622","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Due to their short-range (2–500 nm), Auger electrons (Auger e????) have the potential to\ninduce nano-scale physiochemical damage to biomolecules. Although DNA is the primary target of\nAuger e????, it remains challenging to maximize the interaction between Auger e???? and DNA. To assess\nthe DNA-damaging effect of Auger e???? released as close as possible to DNA without chemical damage,\nwe radio-synthesized no-carrier-added (n.c.a.) [189, 191Pt]cisplatin and evaluated both its in vitro\nproperties and DNA-damaging effect. Cellular uptake, intracellular distribution, and DNA binding\nwere investigated, and DNA double-strand breaks (DSBs) were evaluated by immunofluorescence\nstaining of \nH2AX and gel electrophoresis of plasmid DNA. Approximately 20% of intracellular radio-\nPt was in a nucleus, and about 2% of intra-nucleus radio-Pt bound to DNA, although uptake of n.c.a.\nradio-cisplatin was low (0.6% incubated dose after 25-h incubation), resulting in the frequency of cells\nwith \nH2AX foci was low (1%). Nevertheless, some cells treated with radio-cisplatin had \nH2AX\naggregates unlike non-radioactive cisplatin. These findings suggest n.c.a. radio-cisplatin binding to\nDNA causes severe DSBs by the release of Auger e???? very close to DNA without chemical damage\nby carriers. Efficient radio-drug delivery to DNA is necessary for successful clinical application of\nAuger e????.\nKeywords: Auger electron; cisplatin; 191Pt; 189Pt; radio-drug; DNA double-strand break; \nH2AX","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijms22094622","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.mdpi.com/1422-0067/22/9/4622","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1422-0067","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Honoka, Obata"}],"nameIdentifiers":[{"nameIdentifier":"1015908","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Atsushi, Tsuji"}],"nameIdentifiers":[{"nameIdentifier":"1015909","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hitomi, Sudo"}],"nameIdentifiers":[{"nameIdentifier":"1015910","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aya, Sugyo"}],"nameIdentifiers":[{"nameIdentifier":"1015911","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katsuyuki, Minegishi"}],"nameIdentifiers":[{"nameIdentifier":"1015912","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kotaro, Nagatsu"}],"nameIdentifiers":[{"nameIdentifier":"1015913","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mikako, Ogawa"}],"nameIdentifiers":[{"nameIdentifier":"1015914","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Ming-Rong"}],"nameIdentifiers":[{"nameIdentifier":"1015915","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Honoka, Obata","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1015916","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Atsushi, Tsuji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1015917","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hitomi, Sudo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1015918","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aya, Sugyo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1015919","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katsuyuki, Minegishi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1015920","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kotaro, Nagatsu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1015921","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mikako, Ogawa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1015922","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Ming-Rong","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1015923","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"In Vitro Evaluation of No-Carrier-Added Radiolabeled Cisplatin ([189, 191Pt]cisplatin) Emitting Auger Electrons","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In Vitro Evaluation of No-Carrier-Added Radiolabeled Cisplatin ([189, 191Pt]cisplatin) Emitting Auger Electrons"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-05-06"},"publish_date":"2021-05-06","publish_status":"0","recid":"82791","relation_version_is_last":true,"title":["In Vitro Evaluation of No-Carrier-Added Radiolabeled Cisplatin ([189, 191Pt]cisplatin) Emitting Auger Electrons"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:42:00.426945+00:00"}