{"created":"2023-05-15T15:01:01.151203+00:00","id":82774,"links":{},"metadata":{"_buckets":{"deposit":"f5b94417-c655-42c0-910f-40a29a04bdfa"},"_deposit":{"created_by":1,"id":"82774","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"82774"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00082774","sets":["1"]},"author_link":["1027075","1027079","1027070","1027082","1027074","1027072","1027077","1027080","1027073","1027069","1027081","1027071","1027076","1027078"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"1916","bibliographicPageStart":"1902","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"ChemMedChem"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A series of fluorescent probes from the 6-chloro-2-phenylimidazo[1,2- a ]pyridine-3-yl acetamides ligands featuring the 7-nitro-2-oxa-1,3-diazol-4-yl (NBD) moiety were synthesized and biologically evaluated for their fluorescence properties and for their binding affinity to the 18 kDa translocator protein (TSPO). Spectroscopic studies including UV-Vis absorption and fluorescence measurements showed that the synthesized fluorescent probes exhibit favorable spectroscopic properties, especially in non-polar environments . In vitro fluorescence staining in the brain sections from lipopolysaccharide (LPS)-injected mice revealed partial colocalization of probes with the TSPO. The TSPO binding affinity of probes was measured on crude mitochondrial fractions separated from rat brain homogenates using [ 11 C]PK11195 radioligand binding assay. All the new fluorescent probes demonstrated moderate to high binding affinity to the TSPO, with affinity ( K i ) values ranging from 0.58 nM to 3.28 µM. Taking these data together, we propose that the new fluorescent probes could be used to visualize the TSPO.\n\nKeywords: TSPO; fluorescent probes; imaging; neurodegenerative diseases.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"WILEY"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":" 33631047","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1002/cmdc.202000984","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/cmdc.202000984","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1860-7187","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wongso, Hendris"}],"nameIdentifiers":[{"nameIdentifier":"1027069","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tomoteru, Yamasaki"}],"nameIdentifiers":[{"nameIdentifier":"1027070","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katsushi, Kumata"}],"nameIdentifiers":[{"nameIdentifier":"1027071","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maiko, Ono"}],"nameIdentifiers":[{"nameIdentifier":"1027072","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Makoto, Higuchi"}],"nameIdentifiers":[{"nameIdentifier":"1027073","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Ming-Rong"}],"nameIdentifiers":[{"nameIdentifier":"1027074","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fulham, Michael"}],"nameIdentifiers":[{"nameIdentifier":"1027075","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katsifis, Andrew"}],"nameIdentifiers":[{"nameIdentifier":"1027076","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Anthony Keller, Paul"}],"nameIdentifiers":[{"nameIdentifier":"1027077","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tomoteru, Yamasaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1027078","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katsushi, Kumata","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1027079","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maiko, Ono","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1027080","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Makoto, Higuchi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1027081","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Ming-Rong","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1027082","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Design, Synthesis, and Biological Evaluation of Novel Fluorescent Probes Targeting the 18 kDa Translocator Protein","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Design, Synthesis, and Biological Evaluation of Novel Fluorescent Probes Targeting the 18 kDa Translocator Protein"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-03-03"},"publish_date":"2021-03-03","publish_status":"0","recid":"82774","relation_version_is_last":true,"title":["Design, Synthesis, and Biological Evaluation of Novel Fluorescent Probes Targeting the 18 kDa Translocator Protein"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T17:56:41.372928+00:00"}