@article{oai:repo.qst.go.jp:00082762,
 author = {MORI, YASUMASA and SATO, HIRO and KUMAZAWA, TAKUYA and BUNGA MAYANG PERMATA, TIARA and Yuya, Yoshimoto and Kazutoshi, Murata and SHIN‑EI, NODA and Kaminuma, Takuya and Ken, Ando and OIKE, TAKAHIRO and Noriyuki, Okonogi and Kohei, Okada and KAKOTI, SANGEETA and Keiji, Suzuki and IKOTA, HAYATO and YOKOO, HIDEAKI and Takashi, Nakano and Tatsuya, Ohno and Atsushi, Shibata and Yuya, Yoshimoto and Kazutoshi, Murata and Kaminuma, Takuya and Ken, Ando and Noriyuki, Okonogi and Kohei, Okada and Keiji, Suzuki and Takashi, Nakano and Tatsuya, Ohno and Atsushi, Shibata},
 issue = {6},
 journal = {ONCOLOGY LETTERS},
 month = {Apr},
 note = {Abstract. Radiotherapy induces an immune response in the cancer microenvironment that may influence clinical outcome. The present study aimed to analyse the alteration of CD8+ T‑cell infiltration and programmed death‑ligand 1 (PD‑L1) expression following radiotherapy in clinical samples from patients with uterine cervical squamous cell carcinoma. Additionally, the current study sought to analyse the association between these immune responses and clinical outcomes. A total of 75 patients who received either definitive chemoradiotherapy or radiotherapy were retrospectively analyzed. CD8+ T‑cell infiltration and PD‑L1 expression were determined by immunohistochemistry using biopsy specimens before radiotherapy (pre‑RT) and after 10 Gy radiotherapy (post‑10 Gy). The PD‑L1+ rate was significantly increased from 5% (4/75) pre‑RT to 52% (39/75) post‑10 Gy (P<0.01). Despite this increase in the PD‑L1+ rate post‑10 Gy, there was no significant association between both pre‑RT and post‑10 Gy and overall survival (OS), locoregional control (LC) and progression‑free survival (PFS). On the other hand, the CD8+ T‑cell infiltration density was significantly decreased for all patients (median, 23.1% pre‑RT vs. 16.9% post‑10 Gy; P=0.038); however, this tended to increase in patients treated with radiotherapy alone (median, 17.7% pre‑RT vs. 24.0% post‑10 Gy; P=0.400). Notably, patients with high CD8+ T‑cell infiltration either pre‑RT or post‑10 Gy exhibited positive associations with OS, LC and PFS. Thus, the present analysis suggested that CD8+ T‑cell infiltration may be a prognostic biomarker for patients with cervical cancer receiving radiotherapy. Furthermore, immune checkpoint inhibitors may be effective in patients who have received radiotherapy, since radiotherapy upregulated PD‑L1 expression in cervical cancer specimens.},
 title = {Analysis of radiotherapy‑induced alteration of CD8+ T cells and PD‑L1 expression in patients with uterine cervical squamous cell carcinoma},
 volume = {21},
 year = {2021}
}