{"created":"2023-05-15T15:00:55.016968+00:00","id":82631,"links":{},"metadata":{"_buckets":{"deposit":"86365b78-88a1-450a-ad74-5d5b9d805316"},"_deposit":{"created_by":1,"id":"82631","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"82631"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00082631","sets":["1"]},"author_link":["1013267","1013270","1013266","1013277","1013269","1013275","1013271","1013276","1013265","1013274","1013272","1013264","1013268","1013273"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageStart":"e202000849","bibliographicVolumeNumber":"4","bibliographic_titles":[{"bibliographic_title":"Life Science Alliance"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Infection of certain influenza viruses is triggered when its HA is cleaved by host cell proteases such as proprotein convertases and type II transmembrane serine proteases (TTSP). HA with a monobasic motif is cleaved by trypsin-like proteases, including TMPRSS2 and HAT, whereas the multibasic motif found in high pathogenicity avian influenza HA is cleaved by furin, PC5/6, or MSPL. MSPL belongs to the TMPRSS family and preferentially cleaves [R/K]-K-K-R↓ sequences. Here, we solved the crystal structure of the extracellular region of human MSPL in complex with an irreversible substrate-analog inhibitor. The structure revealed three domains clustered around the C-terminal α-helix of the SPD. The inhibitor structure and its putative model show that the P1-Arg inserts into the S1 pocket, whereas the P2-Lys and P4-Arg interacts with the Asp/Glu-rich 99-loop that is unique to MSPL. Based on the structure of MSPL, we also constructed a homology model of TMPRSS2, which is essential for the activation of the SARS-CoV-2 spike protein and infection. The model may provide the structural insight for the drug development for COVID-19.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Life Science Alliance"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.26508/lsa.202000849","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.life-science-alliance.org/content/4/6/e202000849","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2575-1077","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ayako, Ohno"}],"nameIdentifiers":[{"nameIdentifier":"1013264","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"真板宣夫"}],"nameIdentifiers":[{"nameIdentifier":"1013265","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takanori, Tabata"}],"nameIdentifiers":[{"nameIdentifier":"1013266","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hikaru, Nagano"}],"nameIdentifiers":[{"nameIdentifier":"1013267","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kyohei, Arita"}],"nameIdentifiers":[{"nameIdentifier":"1013268","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mariko, Ariyoshi"}],"nameIdentifiers":[{"nameIdentifier":"1013269","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takayuki, Uchida"}],"nameIdentifiers":[{"nameIdentifier":"1013270","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Reiko, Nakano"}],"nameIdentifiers":[{"nameIdentifier":"1013271","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Anayt, Ulla"}],"nameIdentifiers":[{"nameIdentifier":"1013272","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kosuke, Sugiura"}],"nameIdentifiers":[{"nameIdentifier":"1013273","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koji, Kishimoto"}],"nameIdentifiers":[{"nameIdentifier":"1013274","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Teshima-Kondo, Shigetada"}],"nameIdentifiers":[{"nameIdentifier":"1013275","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yuushi, Okumura"}],"nameIdentifiers":[{"nameIdentifier":"1013276","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nobuo, Maita","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1013277","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Crystal structure of inhibitor-bound human MSPL that can activate high pathogenic avian influenza","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Crystal structure of inhibitor-bound human MSPL that can activate high pathogenic avian influenza"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-04-06"},"publish_date":"2021-04-06","publish_status":"0","recid":"82631","relation_version_is_last":true,"title":["Crystal structure of inhibitor-bound human MSPL that can activate high pathogenic avian influenza"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:53:08.742395+00:00"}