{"created":"2023-05-15T15:00:51.803213+00:00","id":82562,"links":{},"metadata":{"_buckets":{"deposit":"bcdf0aa9-638e-4d22-8004-33ca25d63407"},"_deposit":{"created_by":1,"id":"82562","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"82562"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00082562","sets":["1"]},"author_link":["1008797","1008795","1008799","1008798","1008800","1008796"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"804","bibliographicPageStart":"798","bibliographicVolumeNumber":"83","bibliographic_titles":[{"bibliographic_title":"The Journal of veterinary medical science / the Japanese Society of Veterinary Science"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Radioresistance and radiotoxicity have been reported following cancer treatments in felines. Optimizing radiation doses to induce cytotoxic effects to only cancer cells and not normal cells is critical in achieving effective radiation therapy; however, the mechanisms of radiation resistance, radiotoxicity, and DNA damage response (DDR) in feline cells have not yet been elucidated. A DNA double-strand break (DSB) is the most toxic type of DNA damage induced by X-rays and heavy ion beams used in treating cancers. Crandell-Rees Feline Kidney (CRFK) cells is one of the most widely used cat cells in life science research. Here, we report that DSB-triggered senescence induced by X-rays is important in inhibiting the proliferation of CRFK cells. We demonstrated through cell proliferation assay that X-rays at doses 2 Gy and 10 Gy are toxic to CRFK cells that irradiating CRFK cells inhibits their proliferation. In X-irradiated CRFK cells,\na dose-dependent increase in DSB-triggered senescence was detected according to morphological changes and using senescence-associated β galactosidase staining assay. Moreover, our data indicated that in CRFK cells, the major DDR pathway, which involves the phosphorylation of H2AX at Ser139, was normally activated by ATM kinases. Our findings are useful in the understanding of X-rays-induced cellular senescence and in elucidating biological effects of radiation, e.g., toxicity,\nin feline cells. Furthermore, our findings suggest that the CRFK cell line is an excellent matrix for elucidating radioresistance and radiotoxicity in cat cells.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本獣医学会"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1292/jvms.20-0679","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/jvms/83/5/83_20-0679/_article/-char/ja","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-7250","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Manabu, Koike"}],"nameIdentifiers":[{"nameIdentifier":"1008795","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yasutomo, Yutoku"}],"nameIdentifiers":[{"nameIdentifier":"1008796","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aki, Koike"}],"nameIdentifiers":[{"nameIdentifier":"1008797","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Manabu, Koike","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1008798","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yasutomo, Yutoku","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1008799","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aki, Koike","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1008800","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Inhibition of Crandell-Rees Feline Kidney cell proliferation by X-ray-induced senescence","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Inhibition of Crandell-Rees Feline Kidney cell proliferation by X-ray-induced senescence"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-03-31"},"publish_date":"2021-03-31","publish_status":"0","recid":"82562","relation_version_is_last":true,"title":["Inhibition of Crandell-Rees Feline Kidney cell proliferation by X-ray-induced senescence"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:10:58.263923+00:00"}