@article{oai:repo.qst.go.jp:00082306,
 author = {Asamizu, Shunpei and Ishizuka, Takumi and Yanagisawa, Masaomi and Katsuya, Sato and Yutaka, Ono and Onaka, Hiroyasu and Katsuya, Sato and Yutaka, Ono},
 journal = {QST Takasaki Annual Report 2019},
 month = {Mar},
 note = {Actinomycetes, filamentous Gram-positive bacteria, are a major source of bioactive natural products which can be drug candidates.  We have studied about the bacterial interaction induced production of secondary metabolites by actinomycetes. Tsukamurella pulmonis TP-B0596 (Tp) had been shown to possess ability to induce production of secondary metabolites by Streptomyces species, which are not detected or poorly produced in a mono-culture. Until now, 7 classes, total 29 new compounds had been isolated from the co-culture with various actinomycetes and Tp. Object of this study is to elucidate the gene(s) which are involved in the response for activation of secondary metabolism within actinomycetes. Elucidation of the mechanism can lead to the fundamental understanding of bacterial interaction and secondary metabolism, as well as application for genetic tools to discover novel bioactive natural products from untapped gene matters. Here we employed forward-genetic study using mutagenesis by heavy ion beam to investigated the gene(s) responsible for the responsive production of secondary metabolism induced by Tp. Through this screening, 118 mutants from around 152,000 tested spores were obtained. We further tested the phenotype of the 118 mutants by growth on minimum medium and formation of aerial mycelia, and finally selected 59 mutants. We then re-sequenced the genome of 26 mutants and further gene complementation study revealed that molybdopterin biosynthetic enzyme (moeA), and tetR-like transcriptional regulator are also responsible for the phenotype differences. We speculate that the TetR-like protein is a repressor for the expression of adjacent ABC transporter, thus efflux of the unknown small molecule is prevented and accumulated in the cell, which caused stress to cell that can lead to the undecylprodigiosin production.},
 pages = {101--101},
 title = {Characterization of TetR-type Repressor Which Cause the Delayed Contact-dependent RED Production in Streptomyces coelicolor},
 volume = {QST-M-29},
 year = {2021}
}