{"created":"2023-05-15T15:00:22.351064+00:00","id":81933,"links":{},"metadata":{"_buckets":{"deposit":"0568b632-4e5d-461c-897a-ecea6841471d"},"_deposit":{"created_by":1,"id":"81933","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"81933"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00081933","sets":["1"]},"author_link":["1012743","1012740","1012738","1012741","1012735","1012742","1012736","1012737","1012739"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"77","bibliographicPageStart":"73","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"The Journal of reproduction and development"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Mature mammalian oocytes contain lipid droplets (LDs), which are neutral lipid storage organelles critically important for energy metabolism. In mice, maternal obesity, induced by long-term (> 3 months) high-fat feeding, contributes to the accumulation of LDs in mature oocytes. However, few studies have investigated the influence of short-term high-fat feeding on LD content. In this study, we demonstrated that 3 weeks of high-fat feeding is sufficient to increase LD content and intracellular triacylglycerol levels. Using a two-step centrifugation technique to release LDs into the perivitelline space, we found that short-term high-fat feeding increased the level of LDs in MII oocytes and that 3 days of high-fat feeding were sufficient to increase efficiency of LD release. Collectively, our study suggests that short-term high fat feeding can have a higher impact on lipid metabolism during oocyte maturation.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本繁殖生物学会"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"33071247","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1262/jrd.2020-094","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/jrd/advpub/0/advpub_2020-094/_article/-char/ja/","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-8818","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Aizawa, Ryutaro"}],"nameIdentifiers":[{"nameIdentifier":"1012735","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ibayashi, Megumi"}],"nameIdentifiers":[{"nameIdentifier":"1012736","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hatakeyama, Tomomi"}],"nameIdentifiers":[{"nameIdentifier":"1012737","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tatsumi, Takayuki"}],"nameIdentifiers":[{"nameIdentifier":"1012738","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsukamoto, Satoshi"}],"nameIdentifiers":[{"nameIdentifier":"1012739","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ryutaro, Aizawa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1012740","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Megumi, Ibayashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1012741","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hatakeyama, Tomomi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1012742","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takayuki, Tatsumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1012743","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Impact of short-term high-fat feeding on lipid droplet content in mouse oocytes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Impact of short-term high-fat feeding on lipid droplet content in mouse oocytes"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-11-27"},"publish_date":"2020-11-27","publish_status":"0","recid":"81933","relation_version_is_last":true,"title":["Impact of short-term high-fat feeding on lipid droplet content in mouse oocytes"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:54:49.895819+00:00"}