@article{oai:repo.qst.go.jp:00081933,
 author = {Aizawa, Ryutaro and Ibayashi, Megumi and Hatakeyama, Tomomi and Tatsumi, Takayuki and Tsukamoto, Satoshi and Ryutaro, Aizawa and Megumi, Ibayashi and Hatakeyama, Tomomi and Takayuki, Tatsumi},
 issue = {1},
 journal = {The Journal of reproduction and development},
 month = {Feb},
 note = {Mature mammalian oocytes contain lipid droplets (LDs), which are neutral lipid storage organelles critically important for energy metabolism. In mice, maternal obesity, induced by long-term (> 3 months) high-fat feeding, contributes to the accumulation of LDs in mature oocytes. However, few studies have investigated the influence of short-term high-fat feeding on LD content. In this study, we demonstrated that 3 weeks of high-fat feeding is sufficient to increase LD content and intracellular triacylglycerol levels. Using a two-step centrifugation technique to release LDs into the perivitelline space, we found that short-term high-fat feeding increased the level of LDs in MII oocytes and that 3 days of high-fat feeding were sufficient to increase efficiency of LD release. Collectively, our study suggests that short-term high fat feeding can have a higher impact on lipid metabolism during oocyte maturation.},
 pages = {73--77},
 title = {Impact of short-term high-fat feeding on lipid droplet content in mouse oocytes},
 volume = {67},
 year = {2021}
}