@article{oai:repo.qst.go.jp:00081680, author = {Okamura, Masachika and Hase, Yoshihiro and Hase, Yoshihiro}, journal = {The Carnation Genome}, month = {Dec}, note = {Mutation breeding is advantageous and essential for carnation to extend the variation of genetic information and create novel traits. We have demonstrated that tissue culture combined with ion beam irradiation can create wider spectrum in flower phenotype than that with gamma irradiation, which has been the most commonly used mutagen worldwide. We showed that chronic gamma irradiation also has a potential to generate a wide spectrum of flower color mutants comparable to ion beam irradiation. Furthermore, we found that non-acylated type anthocyanin is the key determinant in creating metallic coloration in carnation. We succeeded in diversifying the metallic coloration by ion beam irradiation. Through these researches, eight new varieties of carnation have been developed so far and sold worldwide for 8 to 15 years. Despite the relatively narrow genetic background of carnation, our research suggested that novel mutant varieties can be created by ion beam breeding system and also by finding key determinants that control flower phenotypes.}, pages = {119--134}, title = {Advances in Mutation Technology to Create Novel Carnation Varieties}, year = {2020} }